产品描述
TSH (促甲状腺激素,thyroid-stimulating hormone) 是垂体腺细胞产生的一种促进甲状腺合成和释放甲状腺素的激素。在人体内,TSH的含量是由甲状腺激素水平负反馈控制的。当甲状腺激素水平下降时,下丘脑和垂体腺释放TRH和TSH来促进甲状腺合成和释放甲状腺素。
华美生物所提供的Rat Thyroid Stimulating Hormone,TSH ELISA Kit属于ELISA检测试剂盒,采用双抗夹心法定量检测大鼠血清、血浆、组织匀浆、细胞裂解液样本中的TSH,其灵敏度为0.3 μIU/mL,检测范围为0.6 μIU/mL-24 μIU/mL。
别名
TshbThyrotropin subunit beta ELISA kit; Thyroid-stimulating hormone subunit beta ELISA kit; TSH-B ELISA kit; TSH-beta ELISA kit; Thyrotropin beta chain ELISA kit
缩写
TSH
Uniprot No
P04652
灵敏度
0.3 μIU/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Signal Transduction
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<15% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<15% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rat TSH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
93 |
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| Range % |
87-98 |
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| 1:2 |
Average % |
87 |
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| Range % |
84-90 |
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| 1:4 |
Average % |
91 |
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| Range % |
85-96 |
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| 1:8 |
Average % |
95 |
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| Range % |
90-99 |
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回收率
| The recovery of rat TSH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
96 |
91-100 |
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| EDTA plasma (n=4) |
93 |
88-99 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| μIU/ml |
OD1 |
OD2 |
Average |
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| 24 |
2.756 |
2.781 |
2.769 |
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| 12 |
1.997 |
1.988 |
1.993 |
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| 6 |
1.142 |
1.124 |
1.133 |
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| 3 |
0.634 |
0.613 |
0.624 |
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| 1.2 |
0.244 |
0.253 |
0.249 |
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| 0.6 |
0.115 |
0.117 |
0.116 |
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货期
3-5 working days