产品描述
甲状腺素(Thyroxine,T4)是一种由甲状腺分泌的激素,在人体内起到调节能量代谢、促进生长发育等多种生理作用。它能影响基础代谢率、心脏功能、中枢神经系统发育和骨骼生长等多个方面,对人体的正常生长、发育和代谢有着重要的作用。甲状腺素水平的异常可以导致甲状腺功能亢进或低下等疾病,严重影响人体健康。
华美生物所提供的Pig Thyroxine(T4) ELISA Kit属于ELISA检测试剂盒,采用竞争法定量检测猪血清、血浆样本中的T4,其灵敏度为20 ng/mL,检测范围为20 ng/mL-320 ng/mL。
缩写
T4
灵敏度
20 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Signal Transduction
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<15% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<15% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of pig T4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
88 |
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| Range % |
82-100 |
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| 1:2 |
Average % |
89 |
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| Range % |
83-98 |
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| 1:4 |
Average % |
95 |
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| Range % |
89-100 |
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| 1:8 |
Average % |
95 |
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| Range % |
90-100 |
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回收率
| The recovery of pig T4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
95 |
90-99 |
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| EDTA plasma (n=4) |
91 |
86-99 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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| ng/ml |
OD1 |
OD2 |
Average |
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| 320 |
0.165 |
0.169 |
0.167 |
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| 160 |
0.279 |
0.272 |
0.276 |
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| 80 |
0.495 |
0.499 |
0.497 |
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| 40 |
1.027 |
1.011 |
1.019 |
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| 20 |
2.052 |
2.031 |
2.042 |
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货期
3-5 working days