产品描述

This Human SDF-1β/SDF1B ELISA Kit was designed for the quantitative measurement of Human SDF-1β/SDF1B protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 125 pg/mL-8000 pg/mL and the sensitivity is 31.25 pg/mL .

别名

12-O-tetradecanoylphorbol 13-acetate repressed protein 1 ELISA Kit; AI174028 ELISA Kit; C-X-C motif chemokine 12 ELISA Kit; Chemokine (C-X-C motif) ligand 12 (stromal cell-derived factor 1) ELISA Kit; Chemokine (C-X-C motif) ligand 12 ELISA Kit; Chemokine CXC motif ligand 12 ELISA Kit; cxcl12 ELISA Kit; hIRH ELISA Kit; hSDF-1 ELISA Kit; Intercrine reduced in hepatomas ELISA Kit; IRH ELISA Kit; OTTHUMP00000019491 ELISA Kit; PBSF ELISA Kit; Pre-B cell growth-stimulating factor ELISA Kit; SCYB12 ELISA Kit; SDF 1 ELISA Kit; SDF-1 ELISA Kit; SDF-1-alpha(3-67) ELISA Kit; SDF-1a ELISA Kit; SDF-1b ELISA Kit; SDF1_HUMAN ELISA Kit; SDF1A ELISA Kit; SDF1B ELISA Kit; Stromal cell-derived factor 1 ELISA Kit; Stromal cell-derived factor 1 delta ELISA Kit; Stromal cell-derived factor 1 gamma ELISA Kit; Stromal cell-derived factor 1a ELISA Kit; Stromal cell-derived factor-1 alpha ELISA Kit; Thymic lymphoma cell-stimulating factor ELISA Kit; Tlsf ELISA Kit; TLSF-a ELISA Kit; TLSF-b ELISA Kit; Tlsfa ELISA Kit; Tlsfb ELISA Kit; TPAR1 ELISA Kit

缩写

SDF-1β/SDF1B

Uniprot No

P48061

灵敏度

31.25 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Immunology

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human SDF1B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		86
		Range %		80-94
1:2		Average %		97
		Range %		92-103
1:4		Average %		98
		Range %		92-105
1:8		Average %		94
		Range %		86-99

回收率

The recovery of human SDF1B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		94		89-99
EDTA plasma (n=4)		98		90-105

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml OD1 OD2 Average Corrected 8000 2.849 2.664 2.757 2.614 4000 2.437 2.291 2.364 2.221 2000 1.677 1.603 1.640 1.497 1000 0.841 0.812 0.827 0.684 500 0.470 0.459 0.465 0.322 250 0.298 0.289 0.294 0.151 125 0.227 0.222 0.225 0.082 0 0.144 0.141 0.143

货期

3-5 working days