产品描述

The human TLR2 ELISA Kit is engineered for accurate measurement of human TLR2 levels from samples including serum, plasma, or tissue homogenates. It uses the Sandwich-ELISA mechanism in combination with the enzyme-substrate chromogenic reaction to measure the TLR2 content in the sample. The color intensity is positively correlated with TLR2 content in the sample. The TLR2 concentration can be calculated according to the standard curve. This kit is tested with high sensitivity, strong specificity, good linearity, high precision and recovery, as well as lot-to-lot consistency.

TLR2 plays a major role in Gram-positive bacterial recognition and defense against mycobacterial infection. TLR2 plays a protective role during infection by triggering a strong pro-inflammatory response, which is beneficial for bacterial clearance. However, the excessive inflammation caused by TLR2 can result in tissue damage and even affect the healing of damaged tissues. It has been reported that TLR2 has a broad range of functions in infectious diseases and also in other diseases, such as chronic and acute inflammatory diseases, cancers, and even metabolic disorders.

别名

CD282 ELISA Kit; CD282 antigen ELISA Kit; TIL 4 ELISA Kit; TIL4 ELISA Kit; TLR 2 ELISA Kit; TLR2 ELISA Kit; TLR2_HUMAN ELISA Kit; Toll like receptor 2 ELISA Kit; Toll like receptor 2 precursor ELISA Kit; Toll-like receptor 2 ELISA Kit; Toll/interleukin 1 receptor like 4 ELISA Kit; Toll/interleukin 1 receptor like protein 4 ELISA Kit; Toll/interleukin receptor like protein 4 ELISA Kit; Toll/interleukin-1 receptor-like protein 4 ELISA Kit

缩写

TLR2

Uniprot No

O60603

灵敏度

0.078 ng/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Immunology

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human TLR2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		96
		Range %		89-101
1:2		Average %		94
		Range %		88-97
1:4		Average %		92
		Range %		85-97
1:8		Average %		94
		Range %		88-98

回收率

The recovery of human TLR2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		83		89-97
EDTA plasma (n=4)		90		85-96

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml OD1 OD2 Average Corrected   20 2.632 2.621 2.627 2.504   10 2.068 2.056 2.062 1.939   5 1.151 1.132 1.142 1.019   2.5 0.673 0.639 0.656 0.533   1.25 0.427 0.424 0.426 0.303   0.625 0.255 0.245 0.250 0.127   0.312 0.189 0.177 0.183 0.060   0 0.125 0.121 0.123

货期

3-5 working days