产品描述
The Rat Pulmonary Surfactant-associated protein D (SP-D) ELISA kit can be your reliable and efficient solution for measuring the levels of Pulmonary surfactant-associated protein D in serum, plasma, and tissue homogenates from Rattus norvegicus (Rat) samples.
With a detection range of 0.312 ng/mL-20 ng/mL and a sensitivity of 0.078 ng/mL, this ELISA kit provides accurate and precise measurements of SP-D levels. The assay time ranges from 1-5 hours and requires a sample volume of 50-100ul, making it convenient and easy to use in your lab.
The assay principle is quantitative and the measurement method is a Sandwich assay, ensuring reliable and reproducible results. The detection wavelength is 450 nm.
Designed for research in Lung-related disease and Signal Transduction, this ELISA kit has been cited in more than 3 publications, attesting to its quality and reliability.
别名
Sftpd ELISA Kit; Sftp4 ELISA Kit; Pulmonary surfactant-associated protein D ELISA Kit; PSP-D ELISA Kit; SP-D ELISA Kit; CP4 ELISA Kit; Lung surfactant protein D ELISA Kit
缩写
SFTPD
Uniprot No
P35248
灵敏度
0.078 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Signal Transduction
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
|
|
|
| Three samples of known concentration were tested twenty times on one plate to assess. |
|
| Inter-assay Precision (Precision between assays): CV%<10% |
|
|
|
| Three samples of known concentration were tested in twenty assays to assess. |
|
|
| |
|
|
|
|
|
|
线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rat SP-D in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| |
Sample |
Serum(n=4) |
|
| 1:5 |
Average % |
88 |
|
| Range % |
82-92 |
|
| 1:10 |
Average % |
94 |
|
| Range % |
90-99 |
|
| 1:20 |
Average % |
95 |
|
| Range % |
91-101 |
|
| 1:40 |
Average % |
94 |
|
| Range % |
90-98 |
|
回收率
| The recovery of rat SP-D spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
89 |
85-96 |
|
| EDTA plasma (n=4) |
92 |
87-98 |
|
| |
|
|
|
|
|
|
| |
|
|
|
|
|
|
标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
|
| 20 |
2.689 |
2.697 |
2.693 |
2.508 |
|
| 10 |
2.001 |
2.054 |
2.028 |
1.843 |
|
| 5 |
1.555 |
1.579 |
1.567 |
1.382 |
|
| 2.5 |
1.112 |
1.165 |
1.139 |
0.954 |
|
| 1.25 |
0.687 |
0.697 |
0.692 |
0.507 |
|
| 0.625 |
0.442 |
0.475 |
0.459 |
0.274 |
|
| 0.312 |
0.382 |
0.396 |
0.389 |
0.204 |
|
| 0 |
0.184 |
0.186 |
0.185 |
|
|
|
货期
3-5 working days
已关闭
固定模块
收藏
易享客服
