产品描述
This Mouse TGFB3 ELISA Kit was designed for the quantitative measurement of Mouse TGFB3 protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 125 pg/mL-8000 pg/mL and the sensitivity is 31.25 pg/mL.
别名
Tgfb3 ELISA Kit; Transforming growth factor beta-3 proprotein [Cleaved into: Latency-associated peptide ELISA Kit; LAP); Transforming growth factor beta-3 ELISA Kit; TGF-beta-3)] ELISA Kit
缩写
TGFB3
Uniprot No
P17125
灵敏度
31.25 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Signal Transduction
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
| | | | | | | |
线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse TGF-β3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 93 | |
| Range % | 89-97 | |
| 1:2 | Average % | 95 | |
| Range % | 92-102 | |
| 1:4 | Average % | 96 | |
| Range % | 93-102 | |
| 1:8 | Average % | 94 | |
| Range % | 89-99 | |
回收率
| The recovery of mouse TGF-β3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 95 | 90-99 | |
| EDTA plasma (n=4) | 91 | 84-98 | |
| | | | | | | |
| | | | | | | |
标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| pg/ml | OD1 | OD2 | Average | Corrected | | | 8000 | 2.646 | 2.815 | 2.731 | 2.542 | | | 4000 | 2.016 | 2.155 | 2.086 | 1.897 | | | 2000 | 1.195 | 1.264 | 1.230 | 1.041 | | | 1000 | 0.778 | 0.798 | 0.788 | 0.599 | | | 500 | 0.493 | 0.506 | 0.500 | 0.311 | | | 250 | 0.336 | 0.361 | 0.349 | 0.160 | | | 125 | 0.279 | 0.265 | 0.272 | 0.083 | | | 0 | 0.192 | 0.185 | 0.189 | | |
|
货期
3-5 working days