产品描述
TGFB1 (Transforming growth factor beta 1) 是一种细胞因子,可以调控多种生物过程,包括细胞增殖、分化、凋亡、免疫反应和胚胎发育等。TGFB1在许多细胞类型中表达,并且是细胞外基质成分之一。TGFB1通过结合细胞膜上的受体使信号转导通路激活,影响下游的转录因子和基因表达。在维持免疫耐受性、肿瘤进展和纤维化等生物学过程中发挥重要作用。TGFB1的含量水平受许多因素影响,如年龄、疾病状态等。
华美生物所提供的Mouse Transforming Growth factor β1,TGF-β1 ELISA kit属于ELISA检测试剂盒,采用双抗夹心法定量检测鼠血清、血浆、细胞培养上清样本中的TGFB1,其灵敏度为0.2 ng/mL,检测范围为0.78 ng/mL-50 ng/mL。
别名
Tgfb1 ELISA Kit; Transforming growth factor beta-1 proprotein [Cleaved into: Latency-associated peptide ELISA Kit; LAP); Transforming growth factor beta-1 ELISA Kit; TGF-beta-1)] ELISA Kit
缩写
TGFB1
Uniprot No
P04202
灵敏度
0.2 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Signal Transduction
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse TGF-β1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
99 |
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| Range % |
94-104 |
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| 1:2 |
Average % |
89 |
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| Range % |
83-97 |
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| 1:4 |
Average % |
92 |
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| Range % |
87-97 |
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| 1:8 |
Average % |
92 |
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| Range % |
87-96 |
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回收率
| The recovery of mouse TGF-β1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
92 |
87-99 |
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| EDTA plasma (n=4) |
96 |
90-100 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 50 |
2.654 |
2.636 |
2.645 |
2.515 |
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| 25 |
2.318 |
2.455 |
2.387 |
2.257 |
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| 12.5 |
1.756 |
1.886 |
1.821 |
1.691 |
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| 6.25 |
0.997 |
1.001 |
0.999 |
0.869 |
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| 3.12 |
0.542 |
0.512 |
0.527 |
0.397 |
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| 1.56 |
0.301 |
0.309 |
0.305 |
0.175 |
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| 0.78 |
0.228 |
0.238 |
0.233 |
0.103 |
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| 0 |
0.129 |
0.131 |
0.130 |
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货期
3-5 working days