产品描述
This Human Pro-ANP ELISA Kit was designed for the quantitative measurement of Human Pro-ANP protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 62.5 pg/mL-4000 pg/mL and the sensitivity is 15.6 pg/mL.
缩写
Pro-ANP
灵敏度
15.6 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cardiovascular
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human Pro-ANP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |
| | Sample | Serum(n=4) | | |
| 1:1 | Average % | 93 | | |
| Range % | 86-97 | | |
| 1:2 | Average % | 85 | | |
| Range % | 80-89 | | |
| 1:4 | Average % | 92 | | |
| Range % | 87-98 | | |
| 1:8 | Average % | 104 | | |
| Range % | 96-108 | | |
回收率
| The recovery of human Pro-ANP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |
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| Sample Type | Average % Recovery | Range | | |
| Serum (n=5) | 89 | 84-95 | | |
| EDTA plasma (n=4) | 87 | 82-93 | | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |
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| pg/ml | OD1 | OD2 | Average | Corrected | | | | 4000 | 1.943 | 1.896 | 1.920 | 1.766 | | | | 2000 | 1.452 | 1.366 | 1.409 | 1.255 | | | | 1000 | 0.998 | 0.968 | 0.983 | 0.829 | | | | 500 | 0.646 | 0.657 | 0.652 | 0.498 | | | | 250 | 0.467 | 0.454 | 0.461 | 0.307 | | | | 125 | 0.322 | 0.306 | 0.314 | 0.160 | | | | 62.5 | 0.209 | 0.203 | 0.206 | 0.052 | | | | 0 | 0.155 | 0.153 | 0.154 | | | |
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货期
3-5 working days