产品描述
This Human PROK1 ELISA Kit was designed for the quantitative measurement of Human PROK1 protein in serum. It is a Sandwich ELISA kit, its detection range is 0.312 ng/mL-20 ng/mL and the sensitivity is 0.078 ng/mL.
别名
Black mamba toxin related protein ELISA Kit; EG VEGF ELISA Kit; EG-VEGF ELISA Kit; EGVEGF ELISA Kit; Endocrine-gland-derived vascular endothelial growth factor ELISA Kit; Mambakine ELISA Kit; PK1 ELISA Kit; PRK1 ELISA Kit; PROK1 ELISA Kit; PROK1_HUMAN ELISA Kit; Prokineticin 1 ELISA Kit; Prokineticin-1 ELISA Kit
缩写
PROK1
Uniprot No
P58294
灵敏度
0.078 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Signal Transduction
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human EG-VEGF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |
| | Sample | Serum(n=4) | | |
| 1:1 | Average % | 103 | | |
| Range % | 100-108 | | |
| 1:2 | Average % | 95 | | |
| Range % | 92-99 | | |
| 1:4 | Average % | 88 | | |
| Range % | 85-92 | | |
| 1:8 | Average % | 99 | | |
| Range % | 96-103 | | |
回收率
| The recovery of human EG-VEGF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |
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| Sample Type | Average % Recovery | Range | | |
| Serum (n=5) | 102 | 100-105 | | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |
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| ng/ml | OD1 | OD2 | Average | Corrected | | | | 20 | 2.625 | 2.741 | 2.683 | 2.558 | | | | 10 | 2.268 | 2.166 | 2.217 | 2.092 | | | | 5 | 1.635 | 1.536 | 1.586 | 1.461 | | | | 2.5 | 1.053 | 1.044 | 1.049 | 0.924 | | | | 1.25 | 0.711 | 0.688 | 0.700 | 0.575 | | | | 0.625 | 0.430 | 0.436 | 0.433 | 0.308 | | | | 0.312 | 0.282 | 0.277 | 0.280 | 0.155 | | | | 0 | 0.121 | 0.129 | 0.125 | | | |
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货期
3-5 working days