产品描述

This Human RAB35 ELISA Kit was designed for the quantitative measurement of Human RAB35 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 47 pg/mL-3000 pg/mL and the sensitivity is 11.7 pg/mL.

别名

GTP binding protein RAY ELISA Kit; GTP-binding protein RAY ELISA Kit; H ray ELISA Kit; OTTHUMP00000240256 ELISA Kit; OTTHUMP00000240257 ELISA Kit; OTTHUMP00000240258 ELISA Kit; OTTHUMP00000240259 ELISA Kit; RAB1C ELISA Kit; Rab35 ELISA Kit; RAB35 member RAS oncogene family ELISA Kit; RAB35; member RAS oncogene family ELISA Kit; RAB35_HUMAN ELISA Kit; Ras related protein rab 1c (GTP binding protein ray) ELISA Kit; Ras related protein Rab 1C ELISA Kit; Ras related protein Rab35 ELISA Kit; Ras-related protein Rab-1C ELISA Kit; Ras-related protein Rab-35 ELISA Kit; RAY ELISA Kit

缩写

RAB35

Uniprot No

Q15286

灵敏度

11.7 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Cell Biology

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human RAB35 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		92
		Range %		89-96
1:2		Average %		95
		Range %		91-99
1:4		Average %		97
		Range %		92-102
1:8		Average %		90
		Range %		85-96

回收率

The recovery of human RAB35 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		96		89-102
EDTA plasma (n=4)		94		90-98

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml OD1 OD2 Average Corrected 3000 2.852 2.752 2.802 2.630 1500 2.200 2.100 2.150 1.978 750 1.371 1.271 1.321 1.149 375 0.790 0.780 0.785 0.613 187.5 0.531 0.521 0.526 0.354 94 0.413 0.403 0.408 0.236 47 0.247 0.237 0.242 0.070 0 0.172 0.171 0.172

货期

3-5 working days