产品描述
This Rabbit sP-selectin ELISA Kit was designed for the quantitative measurement of Rabbit sP-selectin protein in serum, plasma, cell culture supernates, urine, saliva, tissue homogenates. It is a Competitive ELISA kit, its detection range is 31.25 pg/mL-2000 pg/mL and the sensitivity is 7.8 pg/mL .
缩写
sP-selectin
灵敏度
7.8 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Others
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rabbit sP-selectin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:20 | Average % | 98 | |
| Range % | 95-101 | |
| 1:40 | Average % | 96 | |
| Range % | 93-99 | |
| 1:80 | Average % | 87 | |
| Range % | 85-90 | |
| 1:160 | Average % | 92 | |
| Range % | 87-95 | |
回收率
| The recovery of rabbit sP-selectin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 96 | 93-99 | |
| EDTA plasma (n=4) | 101 | 99-104 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| pg/ml | OD1 | OD2 | Average | | | | 2000 | 0.090 | 0.092 | 0.091 | | | | 1000 | 0.135 | 0.126 | 0.131 | | | | 500 | 0.209 | 0.204 | 0.207 | | | | 250 | 0.319 | 0.317 | 0.318 | | | | 125 | 0.457 | 0.446 | 0.452 | | | | 62.5 | 0.581 | 0.598 | 0.590 | | | | 31.25 | 0.812 | 0.836 | 0.824 | | | | 0 | 1.571 | 1.598 | 1.585 | | |
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货期
3-5 working days