产品描述

This Human SMOC2 ELISA Kit was designed for the quantitative measurement of Human SMOC2 protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.156 ng/mL-10 ng/mL and the sensitivity is 0.039 ng/mL.

别名

bA270C4A.1 ELISA Kit; bA37D8.1 (secreted modular calcium binding protein 2) ELISA Kit; bA37D8.1 ELISA Kit; dJ421D16.1 (novel thyroglobulin type 1 repeat containing protein) ELISA Kit; dJ421D16.1 ELISA Kit; MST117 ELISA Kit; MSTP117 ELISA Kit; MSTP140 ELISA Kit; Secreted modular calcium binding protein 2 ELISA Kit; Secreted modular calcium-binding protein 2 ELISA Kit; SMAP 2 ELISA Kit; SMAP-2 ELISA Kit; SMAP2 ELISA Kit; SMOC 2 ELISA Kit; SMOC-2 ELISA Kit; SMOC2 ELISA Kit; SMOC2_HUMAN ELISA Kit; Smooth muscle associated protein 2 ELISA Kit; Smooth muscle-associated protein 2 ELISA Kit; SPARC related modular calcium binding 2 ELISA Kit; SPARC-related modular calcium-binding protein 2 ELISA Kit

缩写

SMOC2

Uniprot No

Q9H3U7

灵敏度

0.039 ng/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Cardiovascular

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human SMOC2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		97
		Range %		92-103
1:2		Average %		100
		Range %		96-104
1:4		Average %		93
		Range %		87-97
1:8		Average %		99
		Range %		92-103

回收率

The recovery of human SMOC2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		91		87-95
EDTA plasma (n=4)		94		88-97

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml OD1 OD2 Average Corrected 10 2.272 2.211 2.242 2.094 5 1.794 1.765 1.780 1.632 2.5 1.190 1.123 1.157 1.009 1.25 0.787 0.754 0.771 0.623 0.625 0.493 0.482 0.488 0.340 0.312 0.336 0.348 0.342 0.194 0.156 0.236 0.221 0.229 0.081 0 0.149 0.147 0.148

货期

3-5 working days