产品描述
SOD1 (Superoxide Dismutase 1) 是一种重要的抗氧化酶,可以降解细胞内的超氧阴离子自由基,维护细胞内稳态,其高含量可提高细胞的抗氧化能力,有利于保护生物体免受氧化应激的伤害。
华美生物所提供的Rat superoxide dismutase 1, soluble (SOD1) ELISA kit属于ELISA检测试剂盒,采用双抗夹心法定量检测大鼠血清、血浆、细胞培养上清、组织匀浆样本中的SOD1,其灵敏度为3.9 pg/mL,检测范围为15.6 pg/mL-1000 pg/mL。
别名
Sod1 ELISA Kit; Superoxide dismutase [Cu-Zn] ELISA Kit; EC 1.15.1.1 ELISA Kit
缩写
SOD1
Uniprot No
P07632
灵敏度
3.9 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Metabolism
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rat SOD1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:200 |
Average % |
93 |
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| Range % |
88-95 |
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| 1:400 |
Average % |
104 |
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| Range % |
100-107 |
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| 1:800 |
Average % |
92 |
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| Range % |
87-95 |
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| 1:1600 |
Average % |
97 |
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| Range % |
92-100 |
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回收率
| The recovery of rat SOD1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
93 |
89-95 |
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| EDTA plasma (n=4) |
95 |
92-97 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 1000 |
2.774 |
2.767 |
2.771 |
2.638 |
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| 500 |
2.226 |
2.305 |
2.266 |
2.133 |
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| 250 |
1.737 |
1.624 |
1.681 |
1.548 |
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| 125 |
1.108 |
1.088 |
1.098 |
0.965 |
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| 62.5 |
0.636 |
0.628 |
0.632 |
0.499 |
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| 31.2 |
0.339 |
0.321 |
0.330 |
0.197 |
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| 15.6 |
0.231 |
0.224 |
0.228 |
0.095 |
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| 0 |
0.130 |
0.136 |
0.133 |
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货期
3-5 working days