产品描述
This Dog PG-E2 ELISA Kit was designed for the quantitative measurement of Dog PG-E2 protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 10 pg/mL-2000 pg/mL and the sensitivity is 5 pg/mL .
缩写
PG-E2
灵敏度
5 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Signal Transduction
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<15% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<15% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of canine PG-E2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 98 | |
| Range % | 90-104 | |
| 1:2 | Average % | 95 | |
| Range % | 89-105 | |
| 1:4 | Average % | 93 | |
| Range % | 87-100 | |
| 1:8 | Average % | 95 | |
| Range % | 90-101 | |
回收率
| The recovery of canine PG-E2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 95 | 85-96 | |
| EDTA plasma (n=4) | 96 | 89-103 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| pg/ml | OD1 | OD2 | Average | | | | 2000 | 0.187 | 0.174 | 0.181 | | | | 500 | 0.320 | 0.315 | 0.318 | | | | 160 | 0.517 | 0.547 | 0.532 | | | | 40 | 0.814 | 0.848 | 0.831 | | | | 10 | 1.316 | 1.374 | 1.345 | | | | 0 | 1.958 | 1.933 | 1.946 | | |
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货期
3-5 working days