产品描述
PROG(孕酮)是一种甾体激素,广泛存在于哺乳动物体内。在雌性体内主要由卵巢合成,是维持妊娠和月经周期正常运转的重要激素之一。PROG也具有一定的神经保护作用和抗炎作用。PROG的浓度随着月经周期、妊娠阶段、年龄、激素替代治疗等因素而变化。
华美生物所提供的Horse Progesterone(PROG) ELISA kit属于ELISA检测试剂盒,采用竞争法定量检测马血清、血浆样本中的PROG,其灵敏度为0.25 ng/mL,检测范围为0.25 ng/mL-100 ng/mL。
缩写
PROG
灵敏度
0.25 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Signal Transduction
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<15% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<15% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of horse PROG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
94 |
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| Range % |
89-107 |
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| 1:2 |
Average % |
89 |
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| Range % |
83-99 |
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| 1:4 |
Average % |
95 |
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| Range % |
85-103 |
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| 1:8 |
Average % |
90 |
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| Range % |
86-102 |
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回收率
| The recovery of horse PROG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
97 |
85-106 |
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| EDTA plasma (n=4) |
95 |
82-99 |
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标准曲线
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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| ng/ml |
OD1 |
OD2 |
Average |
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| 100 |
0.162 |
0.158 |
0.160 |
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| 18.75 |
0.320 |
0.332 |
0.326 |
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| 3.125 |
0.542 |
0.563 |
0.553 |
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| 0.875 |
0.971 |
0.944 |
0.958 |
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| 0.25 |
1.503 |
1.485 |
1.494 |
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| 0 |
2.192 |
2.127 |
2.160 |
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货期
3-5 working days