产品描述

This Human PLAUR ELISA Kit was designed for the quantitative measurement of Human PLAUR protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.625 ng/mL-40 ng/mL and the sensitivity is 0.156 ng/mL .

别名

CD 87 ELISA Kit; CD87 ELISA Kit; CD87 antigen ELISA Kit; MO 3 ELISA Kit; MO3 ELISA Kit; Monocyte activation antigen Mo3 ELISA Kit; Plasminogen activator receptor urokinase ELISA Kit; Plasminogen activator urokinase receptor ELISA Kit; PLAUR ELISA Kit; U PAR ELISA Kit; u plasminogen activator receptor ELISA Kit; U-PAR ELISA Kit; u-plasminogen activator receptor form 2 ELISA Kit; UPA receptor ELISA Kit; uPAR ELISA Kit; UPAR_HUMAN ELISA Kit; Urinary plasminogen activator receptor ELISA Kit; URKR ELISA Kit; Urokinase plasminogen activator receptor ELISA Kit; Urokinase plasminogen activator surface receptor ELISA Kit; urokinase-type plasminogen activator (uPA) receptor ELISA Kit

缩写

PLAUR

Uniprot No

Q03405

灵敏度

0.156 ng/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Cancer

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human uPAR in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		90
		Range %		85-96
1:2		Average %		99
		Range %		92-105
1:4		Average %		89
		Range %		82-98
1:8		Average %		95
		Range %		90-99

回收率

The recovery of human uPAR spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		97		91-104
EDTA plasma (n=4)		92		85-98

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml OD1 OD2 Average Corrected   10 2.549 2.603 2.576 2.451   5 2.058 2.065 2.062 1.937   2.5 1.334 1.422 1.378 1.253   1.25 0.721 0.714 0.718 0.593   0.625 0.461 0.498 0.480 0.355   0.312 0.312 0.326 0.319 0.194   0.156 0.215 0.226 0.221 0.096   0 0.124 0.126 0.125

货期

3-5 working days