产品描述

This Human TRIM63 ELISA Kit was designed for the quantitative measurement of Human TRIM63 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 12.5 pg/mL-800 pg/mL and the sensitivity is 3.1 pg/mL.

别名

E3 ubiquitin-protein ligase TRIM63 ELISA Kit; FLJ32380 ELISA Kit; IRF ELISA Kit; Iris RING finger protein ELISA Kit; MURF 1 ELISA Kit; MURF-1 ELISA Kit; MuRF1 ELISA Kit; MURF2 ELISA Kit; Muscle specific ring finger protein 1 ELISA Kit; Muscle specific ring finger protein 2 ELISA Kit; Muscle-specific RING finger protein 1 ELISA Kit; OTTHUMP00000008701 ELISA Kit; RING finger protein 28 ELISA Kit; RNF 28 ELISA Kit; RNF28 ELISA Kit; SMRZ ELISA Kit; Striated muscle RING zinc finger protein ELISA Kit; TRI63_HUMAN ELISA Kit; TRIM 63 ELISA Kit; Trim63 ELISA Kit; Tripartite motif containing 63 ELISA Kit; tripartite motif containing 63, E3 ubiquitin protein ligase ELISA Kit; Tripartite motif containing protein 63 ELISA Kit; Tripartite motif-containing protein 63 ELISA Kit; Ubiquitin ligase TRIM63 ELISA Kit

缩写

TRIM63

Uniprot No

Q969Q1

灵敏度

3.1 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Cell Biology

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human TRIM63 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:5		Average %		91
		Range %		87-95
1:10		Average %		97
		Range %		93-101
1:20		Average %		102
		Range %		99-104
1:40		Average %		88
		Range %		86-90

回收率

The recovery of human TRIM63 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		90		86-94
EDTA plasma (n=4)		102		98-106

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml OD1 OD2 Average Corrected 800 2.508 2.725 2.617 2.430 400 2.013 2.112 2.063 1.876 200 1.337 1.325 1.331 1.144 100 0.825 0.827 0.826 0.639 50 0.474 0.440 0.457 0.270 25 0.339 0.330 0.335 0.148 12.5 0.271 0.282 0.277 0.090 0 0.184 0.189 0.187

货期

3-5 working days