产品描述

This Human TSG101 ELISA Kit was designed for the quantitative measurement of Human TSG101 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 23.44 pg/mL-1500 pg/mL and the sensitivity is 5.86 pg/mL.

别名

ESCRT I complex subunit TSG101 ELISA Kit; ESCRT-I complex subunit TSG101 ELISA Kit; TS101_HUMAN ELISA Kit; TSG 10 ELISA Kit; TSG 101 ELISA Kit; TSG10 ELISA Kit; Tsg101 ELISA Kit; Tumor susceptibility 101 ELISA Kit; Tumor susceptibility gene 10 ELISA Kit; Tumor susceptibility gene 101 ELISA Kit; Tumor susceptibility gene 101 protein ELISA Kit; Tumor susceptibility protein ELISA Kit; Tumor susceptibility protein isoform 3 ELISA Kit; VPS 23 ELISA Kit; VPS23 ELISA Kit

缩写

TSG101

Uniprot No

Q99816

灵敏度

5.86 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Cancer

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human TSG101 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		98
		Range %		92-104
1:2		Average %		108
		Range %		103-112
1:4		Average %		94
		Range %		90-98
1:8		Average %		89
		Range %		86-95

回收率

The recovery of human TSG101 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		102		99-106
EDTA plasma (n=4)		94		90-98

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected 1500 1.872 1.921 1.897 1.787 750 1.322 1.388 1.355 1.245 375 0.954 0.902 0.928 0.818 187.5 0.608 0.622 0.615 0.505 93.75 0.417 0.412 0.415 0.305 46.88 0.289 0.287 0.288 0.178 23.44 0.230 0.244 0.237 0.127 0 0.109 0.111 0.110 　 .

货期

3-5 working days