产品描述
This Pig VCAM1 ELISA Kit was designed for the quantitative measurement of Pig VCAM1 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 15.6ng/mL-1000ng/mL and the sensitivity is 3.9 ng/mL.
缩写
VCAM1/CD106
灵敏度
3.9 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Signal Transduction
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of pig VCAM-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| Sample | Serum(n=4) | |
| 1:1 | Average % | 92 | |
| Range % | 85-94 | |
| 1:2 | Average % | 94 | |
| Range % | 87-98 | |
| 1:4 | Average % | 103 | |
| Range % | 97-107 | |
| 1:8 | Average % | 101 | |
| Range % | 95-104 | |
回收率
| The recovery of pig VCAM-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 94 | 90-98 | |
| EDTA plasma (n=4) | 89 | 83-94 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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| ng/ml | OD1 | OD2 | Average | Corrected | | | 1000 | 2.335 | 2.178 | 2.257 | 2.100 | | | 500 | 1.574 | 1.475 | 1.525 | 1.368 | | | 250 | 1.007 | 0.982 | 0.995 | 0.838 | | | 125 | 0.591 | 0.586 | 0.589 | 0.432 | | | 62.5 | 0.386 | 0.394 | 0.390 | 0.233 | | | 31.2 | 0.278 | 0.282 | 0.280 | 0.123 | | | 15.6 | 0.228 | 0.225 | 0.227 | 0.070 | | | 0 | 0.158 | 0.156 | 0.157 | | |
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货期
3-5 working days