产品描述

The Human tight junction protein 1 (ZO-1) ELISA kit is a sandwich immunoassay specifically designed and validated for the quantitative detection of ZO-1 in the serum, plasma, cell culture supernates, or cell lysates. It is not intended for diagnostic use. This assay kit was designed and optimized for Tags & Cell Markers research use in humans. The kit has undergone rigorous quality control in multiple parameters, including sensitivity, specificity, precision, linearity, recovery, and inter-batch difference. Refer to the product instructions for more details. This assay employs the quantitative sandwich enzyme immunoassay technique, in which ZO-1 in the samples or standards are sandwiched between pre-coated ZO-1 antibody and Biotin-conjugated antibody specific for ZO-1. HRP-avidin is then added to the wells. Following a wash to remove any unbound reagent, the TMB substrate solution is added to the wells and color develops in proportion to the amount of ZO-1 bound in the initial step. The color development is stopped upon adding the stop solution, and the intensity of the color is measured at 450 nm via a microplate reader. The levels of ZO-1 in the samples are determined by comparing the O.D. (optical density) of the samples to the standard curve.

ZO-1, also called TJP1, is a tight junction (TJ) phosphoprotein acting as a scaffold protein that cross-links and anchors tight junction strand proteins to the actin cytoskeleton. ZO-1 organizes and regulates the structure of tight junctions through direct interactions with transmembrane factors including claudin (CLDN), occludin (OCLN), and junction adhesion molecule A (JAM-A). Besides, ZO-1 shuttles between the nucleus and plasma membrane modulate paracellular permeability and participate in the regulation of gene expression. ZO-1 can also bind to ZONAB through its Src homology 3 (SH3) domain. ZONAB is involved in the control of epithelial cell proliferation. ZO1 was down-regulated in many cancers and decreased ZO1 expression may be closely related to patient prognosis.

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These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml OD1 OD2 Average Corrected 1000 2.484 2.467 2.476 2.329 500 1.707 1.678 1.693 1.546 250 1.076 1.055 1.066 0.919 125 0.679 0.669 0.674 0.527 62.5 0.441 0.436 0.439 0.292 31.2 0.297 0.287 0.292 0.145 15.6 0.222 0.209 0.216 0.069 0 0.149 0.145 0.147

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