产品描述

The human Transthyretin (TTR) ELISA kit is suitable for the quantitative determination of human TTR in different sample types, including serum, plasma, and tissue homogenates. This assay employs the competitive inbition reaction and enzyme-substrate chromogenic reaction to quantify antigen levels in the sample. The amount of synthesized colored products is positively related to the analyte of interest in the sample.

TTR is a tetrameric transport protein that is synthesized in the liver, choroid plexus, and retinal pigment epithelium and then secreted into the bloodstream, cerebrospinal fluid (CSF), and eye, respectively. It is responsible for the transport of the thyroid hormone thyroxine and the RBP bound to retinol (vitamin A). TTR is the key protein in familial amyloid polyneuropathy (FAP), a systemic amyloidosis characterized by the deposition of amyloid derived from mutated TTR. It has recently discovered novel functions of TTR, including neuronal health preservation of both central and peripheral nervous systems, determination of cellular fate, and regulation of proliferation and metabolism.

别名

Amyloid polyneuropathy ELISA Kit; Amyloidosis I ELISA Kit; ATTR ELISA Kit; Carpal tunnel syndrome 1 ELISA Kit; CTS ELISA Kit; CTS1 ELISA Kit; Dysprealbuminemic euthyroidal hyperthyroxinemia ELISA Kit; Dystransthyretinemic hyperthyroxinemia ELISA Kit; Epididymis luminal protein 111 ELISA Kit; HEL111 ELISA Kit; HsT2651 ELISA Kit; PALB ELISA Kit; Prealbumin amyloidosis type I ELISA Kit; Prealbumin ELISA Kit; Prealbumin Thyroxine-binding ELISA Kit; Senile systemic amyloidosis ELISA Kit; TBPA ELISA Kit; Thyroxine binding prealbumin ELISA Kit; Transthyretin ELISA Kit; TTHY_HUMAN ELISA Kit; TTR ELISA Kit; TTR protein ELISA Kit

缩写

TTR

Uniprot No

P02766

灵敏度

0.78 μg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Cardiovascular

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human TTR in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:100		Average %		90
		Range %		87-97
1:200		Average %		97
		Range %		93-104
1:400		Average %		85
		Range %		82-91
1:800		Average %		90
		Range %		85-97

回收率

The recovery of human TTR spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		89		85-93
EDTA plasma (n=4)		92		88-94

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

μg/ml OD1 OD2 Average     25 0.165 0.162 0.164     12.5 0.311 0.305 0.308     6.25 0.612 0.606 0.609     3.12 0.856 0.849 0.853     1.56 1.583 1.575 1.579     0 2.715 2.690 2.703

货期

3-5 working days