产品描述
This Rabbit TIMP1 ELISA Kit was designed for the quantitative measurement of Rabbit TIMP1 protein in serum, plasma, cell culture supernates , tissue homogenates. It is a Competitive ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 7.8 pg/mL.
别名
TIMP1 ELISA Kit; Metalloproteinase inhibitor 1 ELISA Kit; Tissue inhibitor of metalloproteinases 1 ELISA Kit; TIMP-1 ELISA Kit
缩写
TIMP1
Uniprot No
P20614
灵敏度
7.8 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cardiovascular
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rabbit TIMP-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:100 |
Average % |
90 |
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| Range % |
85-97 |
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| 1:200 |
Average % |
102 |
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| Range % |
98-108 |
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| 1:400 |
Average % |
98 |
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| Range % |
94-102 |
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| 1:800 |
Average % |
88 |
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| Range % |
84-93 |
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回收率
| The recovery of rabbit TIMP-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
95 |
89-99 |
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| EDTA plasma (n=4) |
92 |
88-96 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
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| 1000 |
0.086 |
0.084 |
0.085 |
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| 500 |
0.145 |
0.141 |
0.143 |
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| 250 |
0.245 |
0.238 |
0.242 |
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| 125 |
0.392 |
0.413 |
0.403 |
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| 62.5 |
0.725 |
0.727 |
0.726 |
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| 31.2 |
0.963 |
0.944 |
0.954 |
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| 15.6 |
1.154 |
1.179 |
1.167 |
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| 0 |
1.734 |
1.662 |
1.698 |
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货期
3-5 working days