产品描述

This human phospholipidase A2 (PLA2) ELISA kit employs the quantitative sandwich enzyme immunoassay technique to measure the levels of human PLA2 in different samples, including serum, plasma, or tissue homogenates. The enzyme-substrate chromogenic reaction is also used to amplify the signal and quantify the levels of the analyte through the intensity of the colored product. The color intensity positively correlates with the amount of PLA2 bound in the initial step.

PLA2 plays key roles in multiple cellular responses, including phospholipid digestion & metabolism, host defense, and signal transduction. PLA2 catalyzes the hydrolysis of the sn-2 position of membrane glycerophospholipids to liberate arachidonic acid (AA), a precursor of eicosanoids including prostaglandins and leukotrienes. PLA2 is a key enzyme in the regulation of lipid mediators of the inflammatory process. Evidence has shown that PLA2 is involved in all stages of pathogen encounter, from the initial signaling to the uptake, degradation, and presentation of the antigen.

别名

Group IB phospholipase A2 ELISA Kit; Group IB secretory phospholipase A2 ELISA Kit; MGC119834 ELISA Kit; MGC119835 ELISA Kit; PA21B_HUMAN ELISA Kit; Phosphatidylcholine 2 acylhydrolase ELISA Kit; Phosphatidylcholine 2-acylhydrolase 1B ELISA Kit; Phospholipase A2 ELISA Kit; Phospholipase A2 group IB (pancreas) ELISA Kit; Phospholipase A2 group IB ELISA Kit; PLA 2 ELISA Kit; PLA2 ELISA Kit; PLA2A ELISA Kit; PLA2G1B ELISA Kit; PPLA 2 ELISA Kit; PPLA2 ELISA Kit; sPLA2IB ELISA Kit

缩写

PLA2G1B

Uniprot No

P04054

灵敏度

0.11 ng/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Metabolism

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human PLA2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:5		Average %		93
		Range %		88-97
1:10		Average %		104
		Range %		100-109
1:20		Average %		95
		Range %		91-99
1:40		Average %		85
		Range %		81-92

回收率

The recovery of human PLA2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		92		88-96
EDTA plasma (n=4)		97		93-101

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml OD1 OD2 Average Corrected 30 2.534 2.452 2.493 2.384 15 1.652 1.576 1.614 1.505 7.5 1.066 1.004 1.035 0.926 3.75 0.578 0.561 0.570 0.461 1.8 0.387 0.401 0.394 0.285 0.9 0.238 0.245 0.242 0.133 0.45 0.179 0.187 0.183 0.074 0 0.105 0.112 0.109

货期

3-5 working days