产品描述
This Rat TRACP-5b ELISA Kit was designed for the quantitative measurement of Rat TRACP-5b protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.312 mIU/mL-20 mIU/mL and the sensitivity is 0.078 mIU/mL .
缩写
TRACP-5b
灵敏度
0.078 mIU/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Others
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
| | | | | | | |
线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rat TRACP-5b in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 92 | |
| Range % | 89-95 | |
| 1:2 | Average % | 100 | |
| Range % | 97-103 | |
| 1:4 | Average % | 93 | |
| Range % | 88-97 | |
| 1:8 | Average % | 98 | |
| Range % | 93-102 | |
回收率
| The recovery of rat TRACP-5b spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 94 | 90-96 | |
| EDTA plasma (n=4) | 95 | 91-97 | |
| | | | | | | |
| | | | | | | |
标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| mIU/ml | OD1 | OD2 | Average | Corrected | | | 20 | 2.076 | 1.988 | 2.032 | 1.887 | | | 10 | 1.292 | 1.196 | 1.244 | 1.099 | | | 5 | 0.804 | 0.820 | 0.812 | 0.667 | | | 2.5 | 0.511 | 0.521 | 0.516 | 0.371 | | | 1.25 | 0.324 | 0.335 | 0.330 | 0.185 | | | 0.625 | 0.282 | 0.278 | 0.280 | 0.135 | | | 0.312 | 0.223 | 0.212 | 0.218 | 0.073 | | | 0 | 0.146 | 0.144 | 0.145 | | |
|
货期
3-5 working days