产品描述
This rat cyclooxygenase-1 (COX-1) ELISA kit employs the quantitative sandwich enzyme immunoassay technique to measure the levels of rat COX-1 in different samples, including serum, plasma, or tissue homogenates. The enzyme-substrate chromogenic reaction is also used to amplify the signal and quantify the levels of the analyte through the intensity of the colored product. The color intensity positively correlates with the amount of COX-1 bound in the initial step.
COX-1 (PTGS1) is a constitutive enzyme expressed in the vascular endothelium, in platelets, and in many other cells in the body. It is responsible for the initial cyclooxygenase reaction, the target for NSAIDs, the conversion of arachidonic acid to PGG2, and the subsequent peroxidase reaction that converts PGG2 to PGH2. Several studies suggest that COX-1 also plays a role in neuroinflammation. COX-1 also mediates prostaglandin-dependent gastric protection. In gastric mucosa, COX-1 is involved in the synthesis of PGE2 and PGI2, which exert cytoprotective effects on several aspects of gastric function such as an increase of bicarbonate and mucus secretion, reduction of gastric acid and pepsin secretion, and maintaining adequate blood flow to the mucosa.
别名
Ptgs1 ELISA Kit; Cox-1 ELISA Kit; Cox1 ELISA Kit; Prostaglandin G/H synthase 1 ELISA Kit; EC 1.14.99.1 ELISA Kit; Cyclooxygenase-1 ELISA Kit; COX-1 ELISA Kit; Prostaglandin H2 synthase 1 ELISA Kit; PGH synthase 1 ELISA Kit; PGHS-1 ELISA Kit; PHS 1 ELISA Kit; Prostaglandin-endoperoxide synthase 1 ELISA Kit
缩写
PTGS1
Uniprot No
Q63921
灵敏度
1.95 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Metabolism
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
| | | | | | | |
线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rat COX-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:100 | Average % | 86 | |
| Range % | 80-92 | |
| 1:200 | Average % | 92 | |
| Range % | 88-97 | |
| 1:400 | Average % | 93 | |
| Range % | 85-99 | |
| 1:800 | Average % | 101 | |
| Range % | 95-107 | |
回收率
| The recovery of rat COX-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 95 | 89-98 | |
| EDTA plasma (n=4) | 90 | 84-94 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
 | pg/ml | OD1 | OD2 | Average | Corrected | | | 500 | 2.448 | 2.352 | 2.400 | 2.262 | | | 250 | 2.035 | 1.989 | 2.012 | 1.874 | | | 125 | 1.512 | 1.501 | 1.507 | 1.369 | | | 62.5 | 0.922 | 0.879 | 0.901 | 0.763 | | | 31.2 | 0.491 | 0.473 | 0.482 | 0.344 | | | 15.6 | 0.336 | 0.318 | 0.327 | 0.189 | | | 7.8 | 0.207 | 0.199 | 0.203 | 0.065 | | | 0 | 0.139 | 0.137 | 0.138 | | |
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货期
3-5 working days
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