产品描述

This Human PPARA ELISA Kit was designed for the quantitative measurement of Human PPARA protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 78 pg/mL-5000 pg/mL and the sensitivity is 19.5 pg/mL.

别名

hPPAR ELISA Kit; MGC2237 ELISA Kit; MGC2452 ELISA Kit; NR1C1 ELISA Kit; Nuclear receptor subfamily 1 group C member 1 ELISA Kit; OTTHUMP00000197740 ELISA Kit; OTTHUMP00000197741 ELISA Kit; Peroxisome proliferative activated receptor alpha ELISA Kit; Peroxisome proliferator activated receptor alpha ELISA Kit; Peroxisome proliferator-activated receptor alpha ELISA Kit; PPAR ELISA Kit; PPAR-alpha ELISA Kit; ppara ELISA Kit; PPARA_HUMAN ELISA Kit; PPARalpha ELISA Kit

缩写

PPARA

Uniprot No

Q07869

灵敏度

19.5 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Metabolism

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human PPAR-α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		91
		Range %		86-95
1:2		Average %		102
		Range %		97-107
1:4		Average %		91
		Range %		85-97
1:8		Average %		97
		Range %		91-103

回收率

The recovery of human PPAR-α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		95		89-98
EDTA plasma (n=4)		98		90-101

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml OD1 OD2 Average Corrected 5000 2.806 2.869 2.838 2.744 2500 2.070 2.098 2.084 1.990 1250 1.137 1.168 1.153 1.059 625 0.598 0.578 0.588 0.494 312 0.315 0.321 0.318 0.224 156 0.219 0.229 0.224 0.130 78 0.157 0.159 0.158 0.064 0 0.095 0.093 0.094

货期

3-5 working days