产品描述

The human Periostin (POSTN) ELISA Kit is engineered for accurate measurement of human POSTN levels from samples including serum, plasma, or tissue homogenates. It uses the Sandwich-ELISA mechanism in combination with the enzyme-substrate chromogenic reaction to measure the POSTN content in the sample. The color intensity is positively correlated with POSTN content in the sample. The POSTN concentration can be calculated according to the standard curve. This kit is tested with high sensitivity, strong specificity, good linearity, high precision and recovery, as well as lot-to-lot consistency.

POSTN, also called OSF2, is a secreted extracellular matrix (ECM) protein expressed in fibroblasts during bone and teeth development. It plays a dynamic role in mediating the communication between cells and the surrounding microenvironment rather than directly participating in the formation of the ECM. POSTN is also involved in bone density and intervertebral disc (IVD) homeostasis. It also mediates inflammation and reconstruction during bronchial asthma, as well as promotes bone development, reconstruction, repair, and strength. Expression of POSTN has been also associated with the progression and drug resistance of different tumors.

别名

Fasciclin-I like ELISA Kit; MGC119510 ELISA Kit; MGC119511 ELISA Kit; OSF 2 ELISA Kit; OSF-2 ELISA Kit; OSF2 ELISA Kit; Osteoblast specific factor 2 (fasciclin I like) ELISA Kit; Osteoblast specific factor 2 ELISA Kit; Osteoblast specific factor ELISA Kit; Osteoblast-specific factor 2 ELISA Kit; PDLPOSTN ELISA Kit; Periodontal ligament specific periostin ELISA Kit; Periostin ELISA Kit; Periostin isoform thy2 ELISA Kit; Periostin isoform thy4 ELISA Kit; Periostin isoform thy6 ELISA Kit; Periostin isoform thy8 ELISA Kit; Periostin osteoblast specific factor ELISA Kit; PN ELISA Kit; POSTN ELISA Kit; POSTN_HUMAN ELISA Kit; RP11 412K4.1 ELISA Kit

缩写

POSTN

Uniprot No

Q15063

灵敏度

1.56 ng/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Cardiovascular

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human POSTN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:5		Average %		93
		Range %		90-95
1:10		Average %		103
		Range %		99-107
1:20		Average %		94
		Range %		89-97
1:40		Average %		98
		Range %		92-103

回收率

The recovery of human POSTN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		94		89-97
EDTA plasma (n=4)		94		91-97

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml OD1 OD2 Average Corrected 400 2.788 2.803 2.796 2.659 200 2.134 2.154 2.144 2.007 100 1.462 1.516 1.489 1.352 50 0.875 0.839 0.857 0.720 25 0.437 0.424 0.431 0.294 12.5 0.354 0.341 0.348 0.211 6.25 0.245 0.234 0.240 0.103 0 0.138 0.136 0.137

货期

3-5 working days