产品描述

This Human VCP ELISA Kit was designed for the quantitative measurement of Human VCP protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 23.44 pg/mL-1500 pg/mL and the sensitivity is 5.86 pg/mL.

别名

15S Mg(2+) ATPase p97 subunit ELISA Kit; 15S Mg(2+)-ATPase p97 subunit ELISA Kit; ALS14 ELISA Kit; ATPase p97 ELISA Kit; CDC48 ELISA Kit; IBMPFD ELISA Kit; MGC131997 ELISA Kit; MGC148092 ELISA Kit; MGC8560 ELISA Kit; p97 ELISA Kit; TER ATPase ELISA Kit; TERA ELISA Kit; TERA_HUMAN ELISA Kit; Transitional endoplasmic reticulum ATPase ELISA Kit; Valosin containing protein ELISA Kit; Valosin-containing protein ELISA Kit; VCP ELISA Kit; Yeast Cdc48p homolog ELISA Kit

缩写

VCP

Uniprot No

P55072

灵敏度

5.86 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Metabolism

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human VCP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		96
		Range %		89-99
1:2		Average %		101
		Range %		96-104
1:4		Average %		91
		Range %		88-93
1:8		Average %		92
		Range %		86-95

回收率

The recovery of human VCP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		92		87-95
EDTA plasma (n=4)		103		97-105

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml OD1 OD2 Average Corrected 1500 2.072 2.087 2.080 1.913 750 1.404 1.424 1.414 1.247 375 0.812 0.823 0.818 0.651 187.5 0.466 0.448 0.457 0.290 93.75 0.336 0.345 0.341 0.174 46.88 0.272 0.266 0.269 0.102 23.44 0.226 0.217 0.222 0.055 0 0.166 0.168 0.167

货期

3-5 working days