产品描述
This Mouse TREM1 ELISA Kit was designed for the quantitative measurement of Mouse TREM1 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL.
别名
Trem1 ELISA Kit; Triggering receptor expressed on myeloid cells 1 ELISA Kit; TREM-1 ELISA Kit; CD antigen CD354 ELISA Kit
缩写
TREM1
Uniprot No
Q9JKE2
灵敏度
3.9 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse sTREM-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| Sample | Serum(n=4) | |
| 1:1 | Average % | 94 | |
| Range % | 86-99 | |
| 1:2 | Average % | 98 | |
| Range % | 90-102 | |
| 1:4 | Average % | 92 | |
| Range % | 85-96 | |
| 1:8 | Average % | 98 | |
| Range % | 92-101 | |
回收率
| The recovery of mouse sTREM-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 90 | 84-94 | |
| EDTA plasma (n=4) | 100 | 91-105 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| pg/ml | OD1 | OD2 | Average | Corrected | | | 1000 | 2.657 | 2.542 | 2.600 | 2.470 | | | 500 | 2.038 | 2.089 | 2.064 | 1.934 | | | 250 | 1.363 | 1.414 | 1.389 | 1.259 | | | 125 | 0.756 | 0.788 | 0.772 | 0.642 | | | 62.5 | 0.463 | 0.454 | 0.459 | 0.329 | | | 31.3 | 0.321 | 0.313 | 0.317 | 0.187 | | | 15.6 | 0.256 | 0.266 | 0.261 | 0.131 | | | 0 | 0.132 | 0.128 | 0.130 | | |
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货期
3-5 working days