产品描述
This Bovine SOD1 ELISA Kit was designed for the quantitative measurement of Bovine SOD1 protein in serum, plasma. It is a Sandwich ELISA kit, its detection range is 15.6 ng/mL-1000 ng/mL and the sensitivity is 3.9 ng/mL.
别名
SOD1 ELISA Kit; Superoxide dismutase [Cu-Zn] ELISA Kit; EC 1.15.1.1 ELISA Kit
缩写
SOD1
Uniprot No
P00442
灵敏度
3.9 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Metabolism
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of bovine Cu/Zn-SOD in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| Sample | Serum(n=4) | |
| 1:1 | Average % | 97 | |
| Range % | 92-104 | |
| 1:2 | Average % | 91 | |
| Range % | 85-97 | |
| 1:4 | Average % | 90 | |
| Range % | 88-95 | |
| 1:8 | Average % | 100 | |
| Range % | 96-105 | |
回收率
| The recovery of bovine Cu/Zn-SOD spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 90 | 87-94 | |
| EDTA plasma (n=4) | 101 | 103-105 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| ng/ml | OD1 | OD2 | Average | Corrected | | | 1000 | 2.014 | 2.115 | 2.065 | 1.929 | | | 500 | 1.537 | 1.640 | 1.589 | 1.453 | | | 250 | 1.201 | 1.108 | 1.155 | 1.019 | | | 125 | 0.878 | 0.844 | 0.861 | 0.725 | | | 62.5 | 0.541 | 0.555 | 0.548 | 0.412 | | | 31.2 | 0.342 | 0.329 | 0.336 | 0.200 | | | 15.6 | 0.251 | 0.272 | 0.262 | 0.126 | | | 0 | 0.137 | 0.135 | 0.136 | | |
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货期
3-5 working days