产品描述

This human PIN1 ELISA kit employs the quantitative sandwich enzyme immunoassay technique to measure the levels of human PIN1 in multiple samples, including serum, plasma, urine, or tissue homogenates. It also uses the enzyme-substrate chromogenic reaction to visualize and analyze the analyte levels through the color intensity. The intensity of the colored product is in direct proportion to the PIN1 levels in the sample and is measured at 450 nm through a microplate reader.

PIN1 specifically binds and isomerizes the phosphorylated serine/threonine–proline motif, which leads to the alteration of protein structure, function, and stability. The altered structure and function of these phosphorylated proteins regulated by PIN1 are closely related to cancer development. PIN1 participates in cellular processes such as the cell cycle, the folding of newly synthesized proteins, responses to DNA damage and stress, and immune responses. PIN1 is highly expressed in human cancers, including prostate cancer, breast cancer, and oral squamous carcinomas. PIN1 drives tumor progression and is negatively associated with clinical outcomes in patients with cancer.

别名

DOD ELISA Kit; DODO, Drosophila, homolog of ELISA Kit; FLJ40239 ELISA Kit; FLJ77628 ELISA Kit; MGC10717 ELISA Kit; NIMA interacting 1 ELISA Kit; Peptidyl prolyl cis trans isomerase NIMA interacting 1 ELISA Kit; Peptidyl prolyl cis/trans isomerase NIMA interacting ELISA Kit; Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1 ELISA Kit; Peptidyl-prolyl cis-trans isomerase pin1 ELISA Kit; Peptidylprolyl cis/trans isomerase NIMA interacting 1 ELISA Kit; Pin 1 ELISA Kit; Pin1 ELISA Kit; PIN1_HUMAN ELISA Kit; PPIase Pin1 ELISA Kit; Prolyl isomerase ELISA Kit; Protein (peptidylprolyl cis/trans isomerase) NIMA interacting 1 ELISA Kit; Protein NIMA interacting 1 ELISA Kit; Rotamase Pin1 ELISA Kit; UBL 5 ELISA Kit; UBL5 ELISA Kit

缩写

PIN1

Uniprot No

Q13526

灵敏度

0.39 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Neuroscience

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human PIN1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:20		Average %		96
		Range %		92-104
1:40		Average %		98
		Range %		92-104
1:80		Average %		102
		Range %		98-108
1:160		Average %		89
		Range %		83-96

回收率

The recovery of human PIN1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		95		89-99
EDTA plasma (n=4)		101		96-105

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml OD1 OD2 Average Corrected 100 2.563 2.462 2.513 2.388 50 2.003 2.104 2.054 1.929 25 1.500 1.511 1.506 1.381 12.5 1.043 1.054 1.049 0.924 6.25 0.624 0.613 0.619 0.494 3.12 0.371 0.360 0.366 0.241 1.56 0.244 0.238 0.241 0.116 0 0.125 0.124 0.125

货期

3-5 working days