产品描述
This Human TNFSF15 ELISA Kit was designed for the quantitative measurement of Human TNFSF15 protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.78 ng/mL-50 ng/mL and the sensitivity is 0.195 ng/mL.
别名
TNFSF15; TL1; VEGI; Tumor necrosis factor ligand superfamily member 15; TNF ligand-related molecule 1; Vascular endothelial cell growth inhibitor
缩写
TNFSF15
Uniprot No
O95150
灵敏度
0.195 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Others
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human TL1A/TNFSF15 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
90 |
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| Range % |
85-93 |
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| 1:2 |
Average % |
97 |
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| Range % |
92-100 |
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| 1:4 |
Average % |
94 |
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| Range % |
86-97 |
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| 1:8 |
Average % |
100 |
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| Range % |
89-105 |
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回收率
| The recovery of human TL1A/TNFSF15 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
101 |
92-106 |
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| EDTA plasma (n=4) |
101 |
95-104 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 50 |
2.471 |
2.397 |
2.434 |
2.295 |
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| 25 |
1.890 |
1.792 |
1.841 |
1.702 |
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| 12.5 |
1.259 |
1.223 |
1.241 |
1.102 |
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| 6.25 |
0.841 |
0.832 |
0.837 |
0.698 |
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| 3.12 |
0.469 |
0.455 |
0.462 |
0.323 |
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| 1.56 |
0.324 |
0.314 |
0.319 |
0.180 |
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| 0.78 |
0.229 |
0.218 |
0.224 |
0.085 |
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| 0 |
0.141 |
0.137 |
0.139 |
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货期
3-5 working days