产品描述
转铁蛋白(transferrin,TRF)是血浆中主要的含铁蛋白质,主要由肝细胞合成,负责运载由消化管吸收的铁和由红细胞降解释放的铁。以TRF-Fe3+的复合物形式进入骨髓中,供成熟红细胞的生成。血清转铁蛋白测定可反映缺铁性贫血等多种疾病。
华美生物所提供的Human transferrin(TF) ELISA Kit属于ELISA检测试剂盒,采用双抗夹心法定量检测人血清、血浆、尿液、细胞培养上清、脑脊液(CSF)样本中的TF,其灵敏度为0.006 nmol/L,检测范围为0.023 nmol/L-1.5 nmol/L。
别名
Apotransferrin ELISA Kit; Beta 1 metal binding globulin ELISA Kit; Beta-1 metal-binding globulin ELISA Kit; DKFZp781D0156 ELISA Kit; PRO1400 ELISA Kit; PRO1557 ELISA Kit; PRO2086 ELISA Kit; Serotransferrin ELISA Kit; Serotransferrin precursor ELISA Kit; Siderophilin ELISA Kit; TF ELISA Kit; TFQTL1 ELISA Kit; Transferin ELISA Kit; Transferrin ELISA Kit; TRFE_HUMAN ELISA Kit
缩写
TF
Uniprot No
P02787
灵敏度
0.006 nmol/L
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cardiovascular
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human TF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:50000 |
Average % |
89 |
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| Range % |
84-95 |
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| 1:100000 |
Average % |
92 |
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| Range % |
87-97 |
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| 1:200000 |
Average % |
96 |
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| Range % |
90-101 |
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| 1:400000 |
Average % |
90 |
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| Range % |
86-96 |
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回收率
| The recovery of human TF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
96 |
89-101 |
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| EDTA plasma (n=4) |
98 |
93-105 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| nmol/L |
OD1 |
OD2 |
Average |
Corrected |
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| 1.5 |
2.367 |
2.396 |
2.382 |
2.278 |
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| 0.75 |
1.898 |
1.753 |
1.826 |
1.722 |
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| 0.375 |
1.262 |
1.288 |
1.275 |
1.171 |
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| 0.187 |
0.948 |
0.982 |
0.965 |
0.861 |
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| 0.094 |
0.583 |
0.597 |
0.590 |
0.486 |
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| 0.047 |
0.353 |
0.348 |
0.351 |
0.247 |
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| 0.023 |
0.284 |
0.295 |
0.290 |
0.186 |
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| 0 |
0.102 |
0.106 |
0.104 |
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货期
3-5 working days