产品描述

CUSABIO's human BAFF ELISA kit is an in vitro enzyme-linked immunosorbent assay for quantitatively measuring human BAFF in serum, plasma, or tissue homogenates. This assay uses the sandwich enzyme immunoassay technique in combination with the enzyme-substrate chromogenic reaction to quantify the analyte in the sample. The color develops positively to the amount of BAFF in samples. The color intensity is measured at 450 nm via a microplate reader.

BAFF, encoded by the TNFSF13B gene, is a ligand for BAFFR that is required for peripheral B-cell survival and homeostasis. Upon binding to BAFFR, BAFF activates several downstream pathways that regulate basic survival functions including protein synthesis and energy metabolism necessary to extend the half-life of immature, transitional, and mature B cells. BAFF/BAFFR signaling plays a fundamental role during the transition from immature T1 to T2 B cells and therefore in the generation of mature B cells in the spleen. BAFF is increased in autoimmune diseases and is correlated with disease activity. increased levels of BAFF have been detected in the serum and synovial fluid of rheumatoid arthritis (RA) patients.

别名

ApoL related ligand TALL 1 ELISA Kit; B cell Activating Factor ELISA Kit; B lymphocyte stimulator ELISA Kit; B-cell-activating factor ELISA Kit; BAFF ELISA Kit; BLyS ELISA Kit; CD 257 ELISA Kit; CD257 ELISA Kit; CD257 antigen ELISA Kit; Delta BAFF ELISA Kit; Dendritic cell derived TNF like molecule ELISA Kit; Dendritic cell-derived TNF-like molecule ELISA Kit; DTL ELISA Kit; DTL precursor ELISA Kit; PRO738 ELISA Kit; soluble form ELISA Kit; TALL 1 ELISA Kit; TALL-1 ELISA Kit; TALL1 ELISA Kit; THANK ELISA Kit; TN13B_HUMAN ELISA Kit; TNF and APOL related leukocyte expressed ligand 1 ELISA Kit; TNF homolog that activates apoptosis ELISA Kit; TNF homolog that activates apoptosis NKFB and JNK. ELISA Kit; TNF- and APOL-related leukocyte expressed ligand 1 ELISA Kit; TNFSF13B ELISA Kit; TNFSF20 ELISA Kit; TNLG7A ELISA Kit; Tumor necrosis factor (ligand) superfamily member 13b ELISA Kit; Tumor necrosis factor ligand 7A ELISA Kit; Tumor necrosis factor ligand superfamily member 13b ELISA Kit; Tumor necrosis factor ligand superfamily member 20 ELISA Kit; Tumor necrosis factor like protein ZTNF4 ELISA Kit; Tumor necrosis factor superfamily member 13B ELISA Kit; UNQ401 ELISA Kit; ZTNF4 ELISA Kit

缩写

TNFSF13B

Uniprot No

Q9Y275

灵敏度

0.078 ng/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Immunology

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human BAFF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		88
		Range %		82-95
1:2		Average %		90
		Range %		85-96
1:4		Average %		93
		Range %		90-95
1:8		Average %		92
		Range %		88-96

回收率

The recovery of human BAFF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		92		85-99
EDTA plasma (n=4)		90		87-94

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml OD1 OD2 Average Corrected 20 2.912 2.953 2.933 2.819 10 2.142 2.175 2.159 2.045 5 1.295 1.312 1.304 1.190 2.5 0.727 0.695 0.711 0.597 1.25 0.426 0.443 0.435 0.321 0.625 0.297 0.312 0.305 0.191 0.312 0.247 0.261 0.254 0.140 0 0.112 0.115 0.114

货期

3-5 working days