产品描述

This Human PDIA3 ELISA Kit was designed for the quantitative measurement of Human PDIA3 protein in serum, plasma, cell culture supernates. It is a Sandwich ELISA kit, its detection range is 1.25 ng/mL-80 ng/mL and the sensitivity is 0.31 ng/mL.

别名

58 kDa glucose regulated protein ELISA Kit; 58 kDa glucose-regulated protein ELISA Kit; 58 kDa microsomal protein ELISA Kit; Disulfide isomerase ER 60 ELISA Kit; Disulfide isomerase ER-60 ELISA Kit; Endoplasmic reticulum resident protein 57 ELISA Kit; Endoplasmic reticulum resident protein 60 ELISA Kit; ER p57 ELISA Kit; ER protein 57 ELISA Kit; ER protein 60 ELISA Kit; ERp 57 ELISA Kit; ERp57 ELISA Kit; ERp60 ELISA Kit; ERp61 ELISA Kit; Glucose Regulated Protein 58 Kd ELISA Kit; GRP 57 ELISA Kit; GRP 58 ELISA Kit; GRP57 ELISA Kit; HsT17083 ELISA Kit; p58 ELISA Kit; PDIA 3 ELISA Kit; PDIA3 ELISA Kit; PDIA3_HUMAN ELISA Kit; Phospholipase C alpha ELISA Kit; PI PLC ELISA Kit; Protein disulfide isomerase A3 ELISA Kit; Protein disulfide isomerase family A member 3 ELISA Kit; Protein disulfide-isomerase A3 ELISA Kit

缩写

PDIA3

Uniprot No

P30101

灵敏度

0.31 ng/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Others

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human PDIA3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		94
		Range %		87-98
1:2		Average %		104
		Range %		98-108
1:4		Average %		92
		Range %		85-96
1:8		Average %		97
		Range %		91-102

回收率

The recovery of hhuman PDIA3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		90		85-94
EDTA plasma (n=4)		95		90-99

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml OD1 OD2 Average Corrected 80 2.576 2.521 2.549 2.398 40 2.156 2.054 2.105 1.954 20 1.487 1.445 1.466 1.315 10 0.766 0.777 0.772 0.621 5 0.492 0.468 0.480 0.329 2.5 0.374 0.369 0.372 0.221 1.25 0.255 0.248 0.252 0.101 0 0.153 0.149 0.151

货期

3-5 working days