产品描述

This human S100A9 ELISA Kit is suitable for qualitatively determining human concentrations in serum, plasma, and tissue homogenates in vitro. S100A9 is a calcium-binding protein that can be found in cells of myeloid origin, such as monocytes, neutrophils, and dendritic cells. After secretion, S100A9 acts as a chemoattractant to recruit inflammatory cells into the surrounding microenvironment. Secreted S100A9 protein plays a role in creating a favorable environment for cancer growth. It was indicated that myeloid cell-producing S100A9 protein within primary cancers and metastatic sites promotes the accumulation of more myeloid cells, which facilitates the regulation of tumor progression and premetastatic niche formation at metastatic sites. S100A9 has been detected in infiltrating macrophages in rheumatoid arthritis and other inflammatory conditions.

This kit uses the quantitative sandwich-based enzyme immunoassay technique to measure the amount of human S100A9 in the sample. Standards and samples are respectively added to the microplate wells pre-coated with an anti-human S100A9 antibody. Biotin-labeled S100A9 antibody, HRP-avidin, and TMB substrate are pipped into the microplate in turn. The capture antibody pre-coated on the plate captures the S100A9 in the human samples. S100A9 binds to the biotinylated anti-S100A9 human monoclonal antibody. And the biotin on the biotinylated anti-S100A9 human monoclonal antibody binds to the avidin on the enzyme label, forming immune complexes. The color renders blue after the addition of the TMB substrate. The addition of the stop solution into the wells immediately turns the blue into yellow. The concentration of S100A9 in the samples is directly proportional to OD (450nm). Each manufactured lot of this ELISA kit was quality tested for criteria such as sensitivity, specificity, precision, linearity, and lot-to-lot consistency.

别名

Leukocyte L1 complex heavy chain ELISA Kit; 60B8AG ELISA Kit; CAGB ELISA Kit; Calgranulin B ELISA Kit; Calgranulin-B ELISA Kit; Calprotectin L1H subunit ELISA Kit; CFAG ELISA Kit; CGLB ELISA Kit; Cystic fibrosis antigen B ELISA Kit; L1AG ELISA Kit; Leukocyte L1 complex heavy chain ELISA Kit; LIAG ELISA Kit; MAC387 ELISA Kit; MIF ELISA Kit; Migration inhibitory factor related protein 14 ELISA Kit; Migration inhibitory factor-related protein 14 ELISA Kit; MRP 14 ELISA Kit; MRP-14 ELISA Kit; MRP14 ELISA Kit; Myeloid-related protein 14 ELISA Kit; NIF ELISA Kit; OTTHUMP00000015331 ELISA Kit; p14 ELISA Kit; Protein S100-A9 ELISA Kit; S100 A9 ELISA Kit; S100 calcium binding protein A9 ELISA Kit; S100 calcium binding protein A9 calgranulin B ELISA Kit; S100 calcium-binding protein A9 ELISA Kit; S100A9 ELISA Kit; S10A9_HUMAN ELISA Kit

缩写

S100A9

Uniprot No

P06702

灵敏度

1.17 ng/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Immunology

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human S100A9 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		100
		Range %		95-105
1:2		Average %		99
		Range %		94-102
1:4		Average %		94
		Range %		90-99
1:8		Average %		95
		Range %		88-98

回收率

The recovery of human S100A9 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		90		86-95
EDTA plasma (n=4)		96		92-99

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml OD1 OD2 Average Corrected 300 2.615 2.556 2.586 2.445 150 1.841 1.784 1.813 1.672 75 1.263 1.251 1.257 1.116 37.5 0.757 0.739 0.748 0.607 18.75 0.461 0.445 0.453 0.312 9.38 0.288 0.273 0.281 0.140 4.69 0.207 0.198 0.203 0.062 0 0.142 0.140 0.141

货期

3-5 working days