产品描述

This human ATGL ELISA kit employs the quantitative sandwich enzyme immunoassay technique to measure the levels of human ATGL in different samples, including serum, urine, or tissue homogenates. The enzyme-substrate chromogenic reaction is also used to amplify the signal and quantify the levels of the analyte through the intensity of the colored product. The color intensity positively correlates with the amount of ATGL bound in the initial step.

ATGL is a triglyceride lipase that catalyzes the initial step in adipose triglyceride (TG) lipolysis, cooperating with other enzymes to hydrolyze TG to produce fatty acids (FAs) that are crucial energy substrates, precursors for the generation of membrane lipids, and ligands of nuclear receptors. It plays an important role in whole-body energy homeostasis. In addition to mobilizing TG for energy production, ATGL also affects obesity and adipose tissue inflammation, heart failure protection, glucose homeostasis, as well as thermoregulation.

别名

1110001C14Rik ELISA Kit; Adipose triglyceride lipase ELISA Kit; ATGL ELISA Kit; ATGL DESNUTRIN ELISA Kit; Calcium independent phospholipase A2 ELISA Kit; Calcium-independent phospholipase A2 ELISA Kit; Desnutrin ELISA Kit; EC 3.1.1.3 ELISA Kit; FP17548 ELISA Kit; IPLA2 zeta ELISA Kit; IPLA2-zeta ELISA Kit; Mutant patatin like phospholipase domain containing 2 ELISA Kit; Patatin like phospholipase domain containing 2 ELISA Kit; PATATIN LIKE PHOSPHOLIPASE DOMAIN CONTAINING PROTEIN 2 ELISA Kit; Patatin-like phospholipase domain-containing protein 2 ELISA Kit; PEDF R ELISA Kit; PHOSPHOLIPASE A2 CALCIUM INDEPENDENT ZETA ELISA Kit; Pigment epithelium derived factor ELISA Kit; Pigment epithelium-derived factor ELISA Kit; plpl ELISA Kit; plpl2 ELISA Kit; PLPL2_HUMAN ELISA Kit; Pnpla2 ELISA Kit; Transport secretion protein 2 ELISA Kit; Transport secretion protein 2.2 ELISA Kit; Transport-secretion protein 2 ELISA Kit; Triglyceride hydrolase ELISA Kit; TTS 2.2 ELISA Kit; TTS2 ELISA Kit; TTS2.2 ELISA Kit; ZETA ELISA Kit

缩写

PNPLA2

Uniprot No

Q96AD5

灵敏度

1.56 mIU/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Metabolism

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human ATGL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		88
		Range %		82-95
1:2		Average %		96
		Range %		90-100
1:4		Average %		93
		Range %		88-105
1:8		Average %		95
		Range %		82-110

回收率

The recovery of human ATGL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		95		82-103

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

mIU/ml OD1 OD2 Average Corrected 400 2.083 1.992 2.038 1.916 200 1.854 1.796 1.825 1.703 100 1.529 1.484 1.507 1.385 50 1.038 0.976 1.007 0.885 25 0.771 0.709 0.740 0.618 12.5 0.429 0.395 0.412 0.290 6.25 0.286 0.269 0.278 0.156 0 0.124 0.119 0.122

货期

3-5 working days