产品描述

This Human UCHL1 ELISA Kit was designed for the quantitative measurement of Human UCHL1 protein in serum, plasma, tissue homogenates, cerebrospinal fluid (CSF). It is a Sandwich ELISA kit, its detection range is 6.25 ng/mL-400 ng/mL and the sensitivity is 1.56 ng/mL .

别名

Epididymis luminal protein 117 ELISA Kit; Epididymis secretory protein Li 53 ELISA Kit; HEL 117 ELISA Kit; HEL S 53 ELISA Kit; NDGOA ELISA Kit; Neuron cytoplasmic protein 9.5 ELISA Kit; OTTHUMP00000218137 ELISA Kit; OTTHUMP00000218139 ELISA Kit; OTTHUMP00000218140 ELISA Kit; OTTHUMP00000218141 ELISA Kit; Park 5 ELISA Kit; PARK5 ELISA Kit; PGP 9.5 ELISA Kit; PGP9.5 ELISA Kit; PGP95 ELISA Kit; Protein gene product 9.5 ELISA Kit; Ubiquitin C terminal esterase L1 ELISA Kit; Ubiquitin C terminal hydrolase ELISA Kit; Ubiquitin C terminal hydrolase L1 ELISA Kit; Ubiquitin carboxyl terminal esterase L1 ELISA Kit; Ubiquitin carboxyl terminal hydrolase isozyme L1 ELISA Kit; Ubiquitin carboxyl-terminal hydrolase isozyme L1 ELISA Kit; Ubiquitin thioesterase L1 ELISA Kit; Ubiquitin thiolesterase ELISA Kit; Ubiquitin thiolesterase L1 ELISA Kit; UCH-L1 ELISA Kit; UCHL1 ELISA Kit; UCHL1_HUMAN ELISA Kit

缩写

UCHL1

Uniprot No

P09936

灵敏度

1.56 ng/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Cell Biology

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human PGP 9.5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		93
		Range %		90-96
1:2		Average %		98
		Range %		92-105
1:4		Average %		89
		Range %		85-96
1:8		Average %		84
		Range %		80-92

回收率

The recovery of human PGP 9.5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		96		91-101
EDTA plasma (n=4)		93		88-99

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml OD1 OD2 Average Corrected 400 2.269 2.389 2.329 2.235 200 1.778 1.798 1.788 1.694 100 1.241 1.269 1.255 1.161 50 0.726 0.758 0.742 0.648 25 0.414 0.436 0.425 0.331 12.5 0.271 0.255 0.263 0.169 6.25 0.165 0.169 0.167 0.073 0 0.092 0.095 0.094

货期

3-5 working days