产品描述
This Rat SFTPC ELISA Kit was designed for the quantitative measurement of Rat SFTPC protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 4.69 pg/mL-300 pg/mL and the sensitivity is 1.17 pg/mL.
别名
Sftpc ELISA Kit; Sftp2 ELISA Kit; Pulmonary surfactant-associated protein C ELISA Kit; SP-C ELISA Kit; Pulmonary surfactant-associated proteolipid SPL(Val) ELISA Kit
缩写
SFTPC
Uniprot No
P11685
灵敏度
1.17 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Signal Transduction
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rat SP-C in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |
| | Sample | Serum(n=4) | | |
| 1:1000 | Average % | 85 | | |
| Range % | 80-90 | | |
| 1:2000 | Average % | 104 | | |
| Range % | 100-109 | | |
| 1:4000 | Average % | 91 | | |
| Range % | 85-96 | | |
| 1:8000 | Average % | 96 | | |
| Range % | 91-103 | | |
回收率
| The recovery of rat SP-C spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |
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| Sample Type | Average % Recovery | Range | | |
| Serum (n=5) | 98 | 94-102 | | |
| EDTA plasma (n=4) | 87 | 83-91 | | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |
|
| pg/ml | OD1 | OD2 | Average | Corrected | | | | 300 | 2.402 | 2.274 | 2.338 | 2.227 | | | | 150 | 1.939 | 2.051 | 1.995 | 1.884 | | | | 75 | 1.411 | 1.497 | 1.454 | 1.343 | | | | 37.5 | 0.939 | 0.904 | 0.922 | 0.811 | | | | 18.75 | 0.568 | 0.579 | 0.574 | 0.463 | | | | 9.38 | 0.355 | 0.382 | 0.369 | 0.258 | | | | 4.69 | 0.248 | 0.260 | 0.254 | 0.143 | | | | 0 | 0.114 | 0.108 | 0.111 | | | |
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货期
3-5 working days