产品描述
This Chicken TGFB1 ELISA Kit was designed for the quantitative measurement of Chicken TGFB1 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.312 ng/mL-20 ng/mL and the sensitivity is 0.078 ng/mL .
别名
TGFB1 ELISA Kit; Transforming growth factor beta-1 proprotein [Cleaved into: Latency-associated peptide ELISA Kit; LAP); Transforming growth factor beta-1 ELISA Kit; TGF-beta-1)] ELISA Kit
缩写
TGFB1
Uniprot No
P09531
灵敏度
0.078 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Signal Transduction
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of chicken TGF-β1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 93 | |
| Range % | 84-97 | |
| 1:2 | Average % | 95 | |
| Range % | 84-98 | |
| 1:4 | Average % | 96 | |
| Range % | 89-103 | |
| 1:8 | Average % | 95 | |
| Range % | 85-100 | |
回收率
| The recovery of chicken TGF-β1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 90 | 84-97 | |
| EDTA plasma (n=4) | 97 | 92-102 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| ng/ml | OD1 | OD2 | Average | Corrected | | | 20 | 2.011 | 2.105 | 2.058 | 1.965 | | | 10 | 1.790 | 1.828 | 1.809 | 1.716 | | | 5 | 1.466 | 1.527 | 1.497 | 1.404 | | | 2.5 | 1.149 | 1.163 | 1.156 | 1.063 | | | 1.25 | 0.795 | 0.854 | 0.825 | 0.732 | | | 0.625 | 0.433 | 0.425 | 0.429 | 0.336 | | | 0.312 | 0.264 | 0.255 | 0.260 | 0.167 | | | 0 | 0.095 | 0.091 | 0.093 | | |
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货期
3-5 working days