产品描述
This Rat TSG-6 ELISA Kit was designed for the quantitative measurement of Rat TSG-6 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.312 ng/mL-20 ng/mL and the sensitivity is 0.078 ng/mL.
别名
TSG-6, TSG6, hyaluronate-binding protein|tumor necrosis factor alpha-inducible protein 6|tumor necrosis factor-inducible protein 6|tumor necrosis factor-stimulated gene-6 protein
缩写
TSG-6
灵敏度
0.078 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cancer
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
|
|
|
| Three samples of known concentration were tested twenty times on one plate to assess. |
|
| Inter-assay Precision (Precision between assays): CV%<10% |
|
|
|
| Three samples of known concentration were tested in twenty assays to assess. |
|
|
| |
|
|
|
|
|
|
线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rat TSG-6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| |
Sample |
Serum(n=4) |
|
| 1:1 |
Average % |
91 |
|
| Range % |
87-95 |
|
| 1:2 |
Average % |
101 |
|
| Range % |
97-104 |
|
| 1:4 |
Average % |
91 |
|
| Range % |
87-94 |
|
| 1:8 |
Average % |
100 |
|
| Range % |
96-104 |
|
回收率
| The recovery of rat TSG-6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
94 |
91-98 |
|
| EDTA plasma (n=4) |
94 |
91-97 |
|
| |
|
|
|
|
|
|
| |
|
|
|
|
|
|
标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
|
| 20 |
2.545 |
2.532 |
2.539 |
2.373 |
|
| 10 |
1.903 |
1.867 |
1.885 |
1.719 |
|
| 5 |
1.210 |
1.222 |
1.216 |
1.050 |
|
| 2.5 |
0.683 |
0.718 |
0.701 |
0.535 |
|
| 1.25 |
0.429 |
0.448 |
0.439 |
0.273 |
|
| 0.625 |
0.341 |
0.332 |
0.337 |
0.171 |
|
| 0.312 |
0.256 |
0.243 |
0.250 |
0.084 |
|
| 0 |
0.168 |
0.164 |
0.166 |
|
|
|
货期
3-5 working days