产品描述

This Human SMAD4 ELISA Kit was designed for the quantitative measurement of Human SMAD4 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 25 pg/mL-1600 pg/mL and the sensitivity is 6.25 pg/mL.

别名

(Small) Mothers Against Decapentaplegic ELISA Kit; Deleted in Pancreatic Carcinoma 4 ELISA Kit; Deleted in Pancreatic Carcinoma ELISA Kit; Deleted in pancreatic carcinoma locus 4 ELISA Kit; Deletion target in pancreatic carcinoma 4 ELISA Kit; DPC 4 ELISA Kit; DPC4 ELISA Kit; hSMAD4 ELISA Kit; JIP ELISA Kit; MAD homolog 4 ELISA Kit; MAD mothers against decapentaplegic Drosophila homolog 4 ELISA Kit; MAD mothers against decapentaplegic homolog 4 ELISA Kit; MADH 4 ELISA Kit; MADH4 ELISA Kit; Med ELISA Kit; Medea ELISA Kit; Mothers against decapentaplegic homolog 4 ELISA Kit; Mothers against decapentaplegic; Drosophila; homolog of; 4 ELISA Kit; Mothers against DPP homolog 4 ELISA Kit; MYHRS ELISA Kit; OTTHUMP00000163548 ELISA Kit; SMA- and MAD-related protein 4 ELISA Kit; SMAD 4 ELISA Kit; SMAD family member 4 ELISA Kit; SMAD mothers against DPP homolog 4 ELISA Kit; SMAD4 ELISA Kit; SMAD4_HUMAN ELISA Kit

缩写

SMAD4

Uniprot No

Q13485

灵敏度

6.25 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Epigenetics and Nuclear Signaling

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human Smad4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		93
		Range %		89-101
1:2		Average %		92
		Range %		88-100
1:4		Average %		90
		Range %		82-96
1:8		Average %		89
		Range %		82-103

回收率

The recovery of human Smad4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		92		88-96
EDTA plasma (n=4)		95		88-105

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml OD1 OD2 Average Corrected 1600 2.637 2.543 2.590 2.452 800 1.823 1.768 1.796 1.658 400 0.957 0.935 0.946 0.808 200 0.549 0.567 0.558 0.420 100 0.352 0.362 0.357 0.219 50 0.219 0.227 0.223 0.085 25 0.178 0.175 0.177 0.039 0 0.139 0.137 0.138

货期

3-5 working days