产品描述
SELP代表“选择素P”,是一种黏附分子,广泛存在于血小板和内皮细胞表面。SELP可以介导血小板、白细胞和红细胞与内皮细胞的相互作用,从而参与炎症和血栓形成。SELP的含量在许多疾病中都有所变化,例如冠心病、糖尿病和癌症等。测量SELP的含量可以帮助诊断和监测这些疾病。
华美生物所提供的Mouse P-Selectin ELISA kit属于ELISA检测试剂盒,采用双抗夹心法定量检测鼠血清、血浆、组织匀浆样本中的SELP,其灵敏度为7.8 pg/mL,检测范围为31.25 pg/mL-2000 pg/mL。
别名
Selp; Grmp; P-selectin; CD62 antigen-like family member P; Granule membrane protein 140; GMP-140; Leukocyte-endothelial cell adhesion molecule 3; LECAM3; Platelet activation dependent granule-external membrane protein; PADGEM; CD antigen CD62P
缩写
SELP
Uniprot No
Q01102
灵敏度
7.8 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cardiovascular
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse P-Selectin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:100 |
Average % |
92 |
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| Range % |
84-98 |
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| 1:200 |
Average % |
95 |
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| Range % |
81-101 |
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| 1:400 |
Average % |
97 |
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| Range % |
93-103 |
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| 1:800 |
Average % |
95 |
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| Range % |
89-98 |
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回收率
| The recovery of mouse P-Selectin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
96 |
92-99 |
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| EDTA plasma (n=4) |
97 |
95-100 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 2000 |
2.768 |
2.710 |
2.739 |
2.568 |
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| 1000 |
2.263 |
2.224 |
2.244 |
2.073 |
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| 500 |
1.576 |
1.557 |
1.567 |
1.396 |
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| 250 |
1.026 |
1.004 |
1.015 |
0.844 |
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| 125 |
0.595 |
0.611 |
0.603 |
0.432 |
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| 62.5 |
0.441 |
0.438 |
0.440 |
0.269 |
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| 31.25 |
0.289 |
0.271 |
0.280 |
0.109 |
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| 0 |
0.169 |
0.172 |
0.171 |
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货期
3-5 working days