产品描述
This Rat PDGF-DD ELISA Kit was designed for the quantitative measurement of Rat PDGF-DD protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.156 ng/mL-10 ng/mL and the sensitivity is 0.039 ng/mL.
缩写
PDGF-DD
灵敏度
0.039 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Others
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rat PDGF-DD in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 86 | |
| Range % | 80-90 | |
| 1:2 | Average % | 89 | |
| Range % | 85-96 | |
| 1:4 | Average % | 90 | |
| Range % | 84-96 | |
| 1:8 | Average % | 92 | |
| Range % | 86-98 | |
回收率
| The recovery of rat PDGF-DD spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 96 | 92-103 | |
| EDTA plasma (n=4) | 95 | 90-101 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| ng/ml | OD1 | OD2 | Average | Corrected | | | 10 | 2.744 | 2.678 | 2.711 | 2.531 | | | 5 | 2.139 | 2.112 | 2.126 | 1.946 | | | 2.5 | 1.509 | 1.476 | 1.493 | 1.313 | | | 1.25 | 1.025 | 1.003 | 1.014 | 0.834 | | | 0.625 | 0.662 | 0.651 | 0.657 | 0.477 | | | 0.312 | 0.403 | 0.386 | 0.395 | 0.215 | | | 0.156 | 0.292 | 0.272 | 0.282 | 0.102 | | | 0 | 0.181 | 0.179 | 0.180 | | |
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货期
3-5 working days