产品描述
甲状腺素T4是由甲状腺分泌的一种激素,可以调节身体内的代谢率、体温、心率、血压等生理功能。T4可以在体内转化为另一种甲状腺激素T3,而T3则更为活跃,具有更强的生物活性。T4水平的异常会导致甲状腺功能亢进或甲状腺功能减退等疾病。此外,在妊娠期间,T4水平的变化还可能对胎儿的神经发育和生长发育产生影响。
华美生物所提供的Goat Thyroxine(T4) ELISA Kit属于ELISA检测试剂盒,采用竞争法定量检测山羊血清、血浆、组织匀浆样本中的T4,其灵敏度为20 ng/mL,检测范围为20 ng/mL-320 ng/mL。
缩写
T4
灵敏度
20 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Signal Transduction
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<15% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<15% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of goat T4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
93 |
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| Range % |
86-101 |
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| 1:2 |
Average % |
86 |
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| Range % |
81-98 |
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| 1:4 |
Average % |
95 |
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| Range % |
90-100 |
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| 1:8 |
Average % |
95 |
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| Range % |
90-100 |
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回收率
| The recovery of goat T4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
97 |
92-110 |
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| EDTA plasma (n=4) |
95 |
90-105 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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| ng/ml |
OD1 |
OD2 |
Average |
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| 320 |
0.163 |
0.169 |
0.166 |
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| 160 |
0.275 |
0.281 |
0.278 |
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| 80 |
0.493 |
0.502 |
0.498 |
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| 40 |
1.015 |
1.029 |
1.022 |
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| 20 |
2.049 |
2.068 |
2.059 |
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货期
3-5 working days