产品描述
This Rat PLAUR ELISA Kit was designed for the quantitative measurement of Rat PLAUR protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.156 ng/mL-10 ng/mL and the sensitivity is 0.039 ng/mL.
别名
PlaurUrokinase plasminogen activator surface receptor ELISA kit; U-PAR ELISA kit; uPAR ELISA kit; CD antigen CD87 ELISA kit
缩写
PLAUR
Uniprot No
P49616
灵敏度
0.039 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cancer
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rat PLAUR/uPAR in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |
| | Sample | Serum(n=4) | | |
| 1:1 | Average % | 88 | | |
| Range % | 84-92 | | |
| 1:2 | Average % | 103 | | |
| Range % | 98-106 | | |
| 1:4 | Average % | 95 | | |
| Range % | 88-99 | | |
| 1:8 | Average % | 92 | | |
| Range % | 87-96 | | |
回收率
| The recovery of rat PLAUR/uPAR spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |
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| Sample Type | Average % Recovery | Range | | |
| Serum (n=5) | 92 | 83-96 | | |
| EDTA plasma (n=4) | 104 | 96-108 | | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |
|
| ng/ml | OD1 | OD2 | Average | Corrected | | | | 10 | 2.113 | 2.273 | 2.193 | 2.072 | | | | 5 | 1.643 | 1.732 | 1.688 | 1.567 | | | | 2.5 | 1.072 | 1.119 | 1.096 | 0.975 | | | | 1.25 | 0.682 | 0.691 | 0.687 | 0.566 | | | | 0.625 | 0.404 | 0.418 | 0.411 | 0.290 | | | | 0.312 | 0.276 | 0.289 | 0.283 | 0.162 | | | | 0.156 | 0.206 | 0.203 | 0.205 | 0.084 | | | | 0 | 0.120 | 0.121 | 0.121 | | | |
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货期
3-5 working days