产品描述
This Rat VCAM1 ELISA Kit was designed for the quantitative measurement of Rat VCAM1 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 ng/mL-1000 ng/mL and the sensitivity is 3.9 ng/mL.
别名
Vcam1 ELISA Kit; Vcam-1 ELISA Kit; Vascular cell adhesion protein 1 ELISA Kit; V-CAM 1 ELISA Kit; VCAM-1 ELISA Kit; CD antigen CD106 ELISA Kit
缩写
VCAM-1/CD106
Uniprot No
P29534
灵敏度
3.9 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Signal Transduction
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rat VCAM-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 89 | |
| Range % | 85-94 | |
| 1:2 | Average % | 95 | |
| Range % | 86-103 | |
| 1:4 | Average % | 96 | |
| Range % | 82-105 | |
| 1:8 | Average % | 98 | |
| Range % | 87-105 | |
回收率
| The recovery of rat VCAM-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 95 | 89-104 | |
| EDTA plasma (n=4) | 89 | 85-93 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| ng/ml | OD1 | OD2 | Average | Corrected | | | 1000 | 2.614 | 2.704 | 2.659 | 2.525 | | | 500 | 2.145 | 2.236 | 2.191 | 2.057 | | | 250 | 1.516 | 1.536 | 1.526 | 1.392 | | | 125 | 0.863 | 0.848 | 0.856 | 0.722 | | | 62.5 | 0.571 | 0.549 | 0.560 | 0.426 | | | 31.25 | 0.352 | 0.347 | 0.350 | 0.216 | | | 15.6 | 0.234 | 0.244 | 0.239 | 0.105 | | | 0 | 0.135 | 0.132 | 0.134 | | |
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货期
3-5 working days