产品描述
This Human SALL4 ELISA Kit was designed for the quantitative measurement of Human SALL4 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 37.5 pg/mL-2400 pg/mL and the sensitivity is 9.37 pg/mL.
别名
AA407717 ELISA Kit; AL022809 ELISA Kit; AW536104 ELISA Kit; C330011P20Rik ELISA Kit; C78083 ELISA Kit; C78563 ELISA Kit; dJ1112F19.1 ELISA Kit; DRRS ELISA Kit; HSAL4 ELISA Kit; Sal like 4 (Drosophila) ELISA Kit; Sal like 4 ELISA Kit; Sal like Protein 4 ELISA Kit; Sal-like protein 4 ELISA Kit; Sall4 ELISA Kit; SALL4_HUMAN ELISA Kit; Spalt like transcription factor 4 ELISA Kit; Tex20 ELISA Kit; Zinc finger protein 797 ELISA Kit; Zinc finger protein SALL4 ELISA Kit; ZNF797 ELISA Kit
缩写
SALL4
Uniprot No
Q9UJQ4
灵敏度
9.37 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Epigenetics and Nuclear Signaling
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human SALL4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| Sample | Serum(n=4) | |
| 1:1 | Average % | 89 | |
| Range % | 85-92 | |
| 1:2 | Average % | 102 | |
| Range % | 97-107 | |
| 1:4 | Average % | 90 | |
| Range % | 85-95 | |
| 1:8 | Average % | 97 | |
| Range % | 91-103 | |
回收率
| The recovery of human SALL4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 101 | 101-107 | |
| EDTA plasma (n=4) | 98 | 95-101 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| pg/ml | OD1 | OD2 | Average | Corrected | | | 2400 | 2.782 | 2.799 | 2.791 | 2.658 | | | 1200 | 1.921 | 1.965 | 1.943 | 1.810 | | | 600 | 1.082 | 1.098 | 1.090 | 0.957 | | | 300 | 0.540 | 0.564 | 0.552 | 0.419 | | | 150 | 0.335 | 0.337 | 0.336 | 0.203 | | | 75 | 0.243 | 0.251 | 0.247 | 0.114 | | | 37.5 | 0.190 | 0.178 | 0.184 | 0.051 | | | 0 | 0.132 | 0.134 | 0.133 | | |
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货期
3-5 working days