产品描述
睾酮(Testosterone)是一种雄性激素,是由睾丸(在雄性)和卵巢(在雌性)分泌的类固醇激素之一。它对男性性器官和次生性征的发育和维护至关重要,包括生殖器官的发育、身体毛发的分布、声音的变化等。它还在人体中发挥着其他重要的生理作用,如蛋白质合成、骨骼肌质量和力量的维护、红细胞的生产和免疫系统的调节。睾酮的水平通常会随着年龄而下降,在男性和女性身体健康方面有着重要的作用。
华美生物所提供的Canine Testosterone (T) ELISA Kit属于ELISA检测试剂盒,采用竞争法定量检测狗血清、血浆、组织匀浆样本中的T,其灵敏度为0.05 ng/mL,检测范围为0.1 ng/mL-20 ng/mL。
别名
N/A
缩写
T
灵敏度
0.05 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Signal Transduction
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<15% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<15% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of canine testosterone in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
91 |
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| Range % |
86-97 |
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| 1:2 |
Average % |
97 |
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| Range % |
92-103 |
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| 1:4 |
Average % |
85 |
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| Range % |
81-89 |
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| 1:8 |
Average % |
89 |
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| Range % |
83-95 |
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回收率
| The recovery of canine testosterone spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
91 |
87-95 |
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| EDTA plasma (n=4) |
87 |
82-93 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
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| 20 |
0.232 |
0.236 |
0.234 |
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| 5 |
0.435 |
0.436 |
0.436 |
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| 1.6 |
0.611 |
0.613 |
0.612 |
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| 0.4 |
0.984 |
0.978 |
0.981 |
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| 0.1 |
1.401 |
1.403 |
1.402 |
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| 0 |
1.985 |
1.989 |
1.987 |
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货期
3-5 working days