产品描述
The Sheep tri-iodothyronine (T3) ELISA kit is a cutting-edge tool for detecting T3 in a variety of samples, including serum, plasma, cell culture supernates, and tissue homogenates. This ELISA kit is specifically designed for Ovis aries (Sheep) and offers high accuracy, sensitivity, and reliability.
This kit has a detection range of 0.5 ng/mL-8 ng/mL, with a sensitivity of 0.5 ng/mL. It provides a quick and convenient assay time of 1-5 hours, with a sample volume of 50-100ul. The detection wavelength is 450 nm. All these makes it ideal for signal transduction and thyroid function research applications.
This ELISA kit uses a sandwich method of measurement, ensuring accurate and precise detection of T3. It offers unparalleled accuracy, sensitivity, and convenience, making it an essential tool for researchers and scientists studying signal transduction in Ovis aries.
缩写
T3
灵敏度
0.5 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Signal Transduction
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<15% | | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | | |
| Inter-assay Precision (Precision between assays): CV%<15% | | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | | |
| | | | | | | |
线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of sheepT3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |
| | Sample | Serum(n=4) | | |
| 1:1 | Average % | 92 | | |
| Range % | 90-100 | | |
| 1:2 | Average % | 87 | | |
| Range % | 85-96 | | |
| 1:4 | Average % | 95 | | |
| Range % | 90-100 | | |
| 1:8 | Average % | 95 | | |
| Range % | 82-99 | | |
回收率
| The recovery of sheep T3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |
|
| Sample Type | Average % Recovery | Range | | |
| Serum (n=5) | 96 | 85-102 | | |
| EDTA plasma (n=4) | 96 | 90-100 | | |
| | | | | | | |
| | | | | | | |
标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |
|
 | ng/ml | OD1 | OD2 | Average | | | | | 8 | 0.172 | 0.165 | 0.169 | | | | | 4 | 0.289 | 0.278 | 0.284 | | | | | 2 | 0.549 | 0.557 | 0.553 | | | | | 1 | 1.252 | 1.263 | 1.258 | | | | | 0.5 | 2.124 | 2.141 | 2.133 | | | |
|
货期
3-5 working days
已关闭
固定模块
收藏
易享客服
